Capnocytophaga felis sp. nov. isolated from the feline oral cavity

Four strains, KC07070 T , KC07105, 11 025B-8C and 11 026B-8-C, were isolated from the oral cavity of cats in 2007 or 2011 in Japan. These strains were Gram-stain-negative rods, exhibited gliding motility, grew in air with 5 % CO 2 and showed catalase and oxidase activity. The sequences of 16S rRNA g...

Full description

Saved in:
Bibliographic Details
Published inInternational journal of systematic and evolutionary microbiology Vol. 70; no. 5; pp. 3355 - 3360
Main Authors Suzuki, Michio, Umeda, Kaoru, Kimura, Masanobu, Imaoka, Koichi, Morikawa, Shigeru, Maeda, Ken
Format Journal Article
LanguageEnglish
Published England 01.05.2020
Subjects
oral cavity
Capnocytophaga
feline
Online AccessGet full text

Cover

Abstract Four strains, KC07070 T , KC07105, 11 025B-8C and 11 026B-8-C, were isolated from the oral cavity of cats in 2007 or 2011 in Japan. These strains were Gram-stain-negative rods, exhibited gliding motility, grew in air with 5 % CO 2 and showed catalase and oxidase activity. The sequences of 16S rRNA genes of the four strains were 100 % identical. Additionally, the sequences of 16S rRNA genes of KC07070 T had identity to those of the type strains of Capnocytophaga canimorsus (97.7 %), Capnocytophaga cynodegmi (97.8 %) and Capnocytophaga canis (97.4 %) and 91.2–93.8% identity to those of other species of the genus Capnocytophaga . The major cellular fatty acids of KC07070 T were iso-C 15 : 0 (56.2 %) and summed feature 11 (14.9 %). The G+C content of the DNA from KC07070 T was 35.6 mol%, and the genome size was 2.88 Mbp. KC07070 T had digital DNA–DNA hybridization (dDDH) values of 26.2–27.6% and average nucleotide identity (ANI) values of 75.4–83.3 % to the type strains of the closest relatives, C. canimorsus , C. cynodegmi and C. canis . These results of phylogenetic analysis of 16S rRNA gene sequence, cellular fatty acids compositions and dDDH and ANI values indicate that strain KC07070 T represents a novel species, for which we propose the name Capnocytophaga felis sp. nov., with type strain KC07070 T (=JCM 32681 T =DSM 107251 T ).
AbstractList Four strains, KC07070T, KC07105, 11 025B-8C and 11 026B-8-C, were isolated from the oral cavity of cats in 2007 or 2011 in Japan. These strains were Gram-stain-negative rods, exhibited gliding motility, grew in air with 5 % CO2 and showed catalase and oxidase activity. The sequences of 16S rRNA genes of the four strains were 100 % identical. Additionally, the sequences of 16S rRNA genes of KC07070T had identity to those of the type strains of Capnocytophaga canimorsus (97.7 %), Capnocytophaga cynodegmi (97.8 %) and Capnocytophaga canis (97.4 %) and 91.2-93.8% identity to those of other species of the genus Capnocytophaga. The major cellular fatty acids of KC07070T were iso-C15 : 0 (56.2 %) and summed feature 11 (14.9 %). The G+C content of the DNA from KC07070T was 35.6 mol%, and the genome size was 2.88 Mbp. KC07070T had digital DNA-DNA hybridization (dDDH) values of 26.2-27.6% and average nucleotide identity (ANI) values of 75.4-83.3 % to the type strains of the closest relatives, C. canimorsus, C. cynodegmi and C. canis. These results of phylogenetic analysis of 16S rRNA gene sequence, cellular fatty acids compositions and dDDH and ANI values indicate that strain KC07070T represents a novel species, for which we propose the name Capnocytophaga felis sp. nov., with type strain KC07070T (=JCM 32681T=DSM 107251T).Four strains, KC07070T, KC07105, 11 025B-8C and 11 026B-8-C, were isolated from the oral cavity of cats in 2007 or 2011 in Japan. These strains were Gram-stain-negative rods, exhibited gliding motility, grew in air with 5 % CO2 and showed catalase and oxidase activity. The sequences of 16S rRNA genes of the four strains were 100 % identical. Additionally, the sequences of 16S rRNA genes of KC07070T had identity to those of the type strains of Capnocytophaga canimorsus (97.7 %), Capnocytophaga cynodegmi (97.8 %) and Capnocytophaga canis (97.4 %) and 91.2-93.8% identity to those of other species of the genus Capnocytophaga. The major cellular fatty acids of KC07070T were iso-C15 : 0 (56.2 %) and summed feature 11 (14.9 %). The G+C content of the DNA from KC07070T was 35.6 mol%, and the genome size was 2.88 Mbp. KC07070T had digital DNA-DNA hybridization (dDDH) values of 26.2-27.6% and average nucleotide identity (ANI) values of 75.4-83.3 % to the type strains of the closest relatives, C. canimorsus, C. cynodegmi and C. canis. These results of phylogenetic analysis of 16S rRNA gene sequence, cellular fatty acids compositions and dDDH and ANI values indicate that strain KC07070T represents a novel species, for which we propose the name Capnocytophaga felis sp. nov., with type strain KC07070T (=JCM 32681T=DSM 107251T).
Four strains, KC07070 T , KC07105, 11 025B-8C and 11 026B-8-C, were isolated from the oral cavity of cats in 2007 or 2011 in Japan. These strains were Gram-stain-negative rods, exhibited gliding motility, grew in air with 5 % CO 2 and showed catalase and oxidase activity. The sequences of 16S rRNA genes of the four strains were 100 % identical. Additionally, the sequences of 16S rRNA genes of KC07070 T had identity to those of the type strains of Capnocytophaga canimorsus (97.7 %), Capnocytophaga cynodegmi (97.8 %) and Capnocytophaga canis (97.4 %) and 91.2–93.8% identity to those of other species of the genus Capnocytophaga . The major cellular fatty acids of KC07070 T were iso-C 15 : 0 (56.2 %) and summed feature 11 (14.9 %). The G+C content of the DNA from KC07070 T was 35.6 mol%, and the genome size was 2.88 Mbp. KC07070 T had digital DNA–DNA hybridization (dDDH) values of 26.2–27.6% and average nucleotide identity (ANI) values of 75.4–83.3 % to the type strains of the closest relatives, C. canimorsus , C. cynodegmi and C. canis . These results of phylogenetic analysis of 16S rRNA gene sequence, cellular fatty acids compositions and dDDH and ANI values indicate that strain KC07070 T represents a novel species, for which we propose the name Capnocytophaga felis sp. nov., with type strain KC07070 T (=JCM 32681 T =DSM 107251 T ).
Four strains, KC07070 , KC07105, 11 025B-8C and 11 026B-8-C, were isolated from the oral cavity of cats in 2007 or 2011 in Japan. These strains were Gram-stain-negative rods, exhibited gliding motility, grew in air with 5 % CO and showed catalase and oxidase activity. The sequences of 16S rRNA genes of the four strains were 100 % identical. Additionally, the sequences of 16S rRNA genes of KC07070 had identity to those of the type strains of (97.7 %), (97.8 %) and (97.4 %) and 91.2-93.8% identity to those of other species of the genus . The major cellular fatty acids of KC07070 were iso-C (56.2 %) and summed feature 11 (14.9 %). The G+C content of the DNA from KC07070 was 35.6 mol%, and the genome size was 2.88 Mbp. KC07070 had digital DNA-DNA hybridization (dDDH) values of 26.2-27.6% and average nucleotide identity (ANI) values of 75.4-83.3 % to the type strains of the closest relatives, , and . These results of phylogenetic analysis of 16S rRNA gene sequence, cellular fatty acids compositions and dDDH and ANI values indicate that strain KC07070 represents a novel species, for which we propose the name sp. nov., with type strain KC07070 (=JCM 32681 =DSM 107251 ).
Author Umeda, Kaoru
Morikawa, Shigeru
Suzuki, Michio
Kimura, Masanobu
Imaoka, Koichi
Maeda, Ken
Author_xml – sequence: 1
  givenname: Michio
  orcidid: 0000-0002-1653-6251
  surname: Suzuki
  fullname: Suzuki, Michio
  organization: Department of Veterinary Science, National Institute of Infectious Diseases, Tokyo 162-8640, Japan
– sequence: 2
  givenname: Kaoru
  surname: Umeda
  fullname: Umeda, Kaoru
  organization: Division of Microbiology, Osaka Institute of Public Health, Osaka 543-0026, Japan
– sequence: 3
  givenname: Masanobu
  surname: Kimura
  fullname: Kimura, Masanobu
  organization: Department of Veterinary Science, National Institute of Infectious Diseases, Tokyo 162-8640, Japan
– sequence: 4
  givenname: Koichi
  surname: Imaoka
  fullname: Imaoka, Koichi
  organization: Department of Veterinary Science, National Institute of Infectious Diseases, Tokyo 162-8640, Japan
– sequence: 5
  givenname: Shigeru
  orcidid: 0000-0001-6249-6716
  surname: Morikawa
  fullname: Morikawa, Shigeru
  organization: Faculty of Veterinary Medicine, Okayama University of Science, Ehime 794-8555, Japan
– sequence: 6
  givenname: Ken
  surname: Maeda
  fullname: Maeda, Ken
  organization: Department of Veterinary Science, National Institute of Infectious Diseases, Tokyo 162-8640, Japan
BackLink https://www.ncbi.nlm.nih.gov/pubmed/32375938$$D View this record in MEDLINE/PubMed
BookMark eNp1kDtPwzAURi1URB-wMiKPLAl27Dw8QsVLqsQCs3Xr2NRVEgfbrdR_T2hLBySm--nqfFe6Z4pGnes0QteUpJQIcWfXQbcpSQnhtCzO0ITyokhywvjolLNijKYhrAkZFoRcoDHLWJkLVk3Qwxz6zqlddP0KPgEb3diAQ5_izm1TbINrIOoaG-9aHFd6D3QaOw8NVrC1cXeJzg00QV8d5wx9PD2-z1-Sxdvz6_x-kShW0JgYyjOzXNYA1GSGq9wUoqS1YDVoSkvFWV5VjORQAhH5EipDSk0rAXVheKlzNkO3h7u9d18bHaJsbVC6aaDTbhNkxoSoGCU0G9CbI7pZtrqWvbct-J38_XsA0gOgvAvBa3NCKJE_YuVerCTyIHYo8D8FZSNE67rowTb_1b4B2C585A
CitedBy_id crossref_primary_10_1099_ijsem_0_005731
crossref_primary_10_1007_s10096_024_05025_0
crossref_primary_10_1007_s10096_022_04402_x
crossref_primary_10_2169_internalmedicine_7947_21
crossref_primary_10_1099_ijsem_0_004979
crossref_primary_10_1128_mra_00912_24
crossref_primary_10_1128_jcm_00281_22
Cites_doi 10.1093/molbev/msv150
10.1128/genomeA.01186-17
10.1007/s10096-015-2360-7
10.1007/BF00408040
10.1128/JCM.27.2.231-235.1989
10.1016/j.vetmic.2014.03.023
10.1093/bioinformatics/btx324
10.1038/srep22919
10.1007/s00284-017-1397-5
10.1093/bioinformatics/btv681
10.1038/s41467-019-10210-3
10.1086/282802
10.1099/00207713-44-2-324
10.1016/j.vetmic.2010.01.001
10.1093/molbev/msw054
10.1186/1471-2105-14-60
10.1093/oxfordjournals.molbev.a040454
10.1016/j.vetmic.2009.01.040
10.1038/emi.2015.48
10.1111/1348-0421.12642
10.1073/pnas.0404206101
10.1111/j.1558-5646.1985.tb00420.x
10.1099/ijs.0.65373-0
10.1128/JB.01962-12
10.1093/bioinformatics/btx713
ContentType Journal Article
DBID AAYXX
CITATION
NPM
7X8
DOI 10.1099/ijsem.0.004176
DatabaseName CrossRef
PubMed
MEDLINE - Academic
DatabaseTitle CrossRef
PubMed
MEDLINE - Academic
DatabaseTitleList MEDLINE - Academic
CrossRef
PubMed
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
Discipline Biology
EISSN 1466-5034
EndPage 3360
ExternalDocumentID 32375938
10_1099_ijsem_0_004176
Genre Journal Article
GroupedDBID ---
-~X
18M
2WC
4.4
53G
5GY
AAYXX
ABDNZ
ACGFO
ACPEE
ADBBV
ADCDP
AEILP
AENEX
AJKYU
ALMA_UNASSIGNED_HOLDINGS
BAWUL
CITATION
CS3
DIK
DU5
E3Z
EBS
EJD
F5P
FRP
GX1
H13
L7B
OK1
P0W
P2P
RGM
TR2
W8F
WH7
WOQ
YSK
~02
.GJ
34G
39C
ABEFU
ACBNA
ACYGS
AGCDD
AI.
C1A
NPM
OHT
VH1
Y6R
ZCG
~KM
7X8
ID FETCH-LOGICAL-c361t-f142fbbdaa1f2f4c5f6971d93dae117c43588305a7a095ba8f07e189ad6f47e53
ISSN 1466-5026
1466-5034
IngestDate Fri Jul 11 04:58:16 EDT 2025
Wed Feb 19 02:29:52 EST 2025
Tue Jul 01 01:30:33 EDT 2025
Thu Apr 24 22:57:31 EDT 2025
IsPeerReviewed true
IsScholarly true
Issue 5
Keywords oral cavity
Capnocytophaga
feline
Language English
LinkModel OpenURL
MergedId FETCHMERGED-LOGICAL-c361t-f142fbbdaa1f2f4c5f6971d93dae117c43588305a7a095ba8f07e189ad6f47e53
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ORCID 0000-0001-6249-6716
0000-0002-1653-6251
PMID 32375938
PQID 2399831012
PQPubID 23479
PageCount 6
ParticipantIDs proquest_miscellaneous_2399831012
pubmed_primary_32375938
crossref_primary_10_1099_ijsem_0_004176
crossref_citationtrail_10_1099_ijsem_0_004176
ProviderPackageCode CITATION
AAYXX
PublicationCentury 2000
PublicationDate 2020-May
PublicationDateYYYYMMDD 2020-05-01
PublicationDate_xml – month: 05
  year: 2020
  text: 2020-May
PublicationDecade 2020
PublicationPlace England
PublicationPlace_xml – name: England
PublicationTitle International journal of systematic and evolutionary microbiology
PublicationTitleAlternate Int J Syst Evol Microbiol
PublicationYear 2020
References Sesser (R23) 1990
R21
R20
R22
R25
R24
R27
R26
R1
R2
R3
R4
R5
R6
R7
R8
R9
R10
Lane (R11) 1991
R12
R14
R13
R16
R15
R17
Richter (R18) 2016; 32
R19
References_xml – ident: R20
  doi: 10.1093/molbev/msv150
– ident: R27
  doi: 10.1128/genomeA.01186-17
– ident: R9
  doi: 10.1007/s10096-015-2360-7
– start-page: 115
  volume-title: Nucleic Acid Techniques in Bacterial Systematics
  year: 1991
  ident: R11
– volume-title: Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids
  year: 1990
  ident: R23
– ident: R1
  doi: 10.1007/BF00408040
– ident: R2
  doi: 10.1128/JCM.27.2.231-235.1989
– ident: R24
  doi: 10.1016/j.vetmic.2014.03.023
– ident: R22
  doi: 10.1093/bioinformatics/btx324
– ident: R26
  doi: 10.1038/srep22919
– ident: R6
  doi: 10.1007/s00284-017-1397-5
– volume: 32
  start-page: 929
  year: 2016
  ident: R18
  article-title: JSpeciesWS: a web server for prokaryotic species circumscription based on pairwise genome comparison
  publication-title: Bioinformatics
  doi: 10.1093/bioinformatics/btv681
– ident: R19
  doi: 10.1038/s41467-019-10210-3
– ident: R21
  doi: 10.1086/282802
– ident: R3
  doi: 10.1099/00207713-44-2-324
– ident: R8
  doi: 10.1016/j.vetmic.2010.01.001
– ident: R12
  doi: 10.1093/molbev/msw054
– ident: R17
  doi: 10.1186/1471-2105-14-60
– ident: R13
  doi: 10.1093/oxfordjournals.molbev.a040454
– ident: R7
  doi: 10.1016/j.vetmic.2009.01.040
– ident: R5
  doi: 10.1038/emi.2015.48
– ident: R10
  doi: 10.1111/1348-0421.12642
– ident: R14
  doi: 10.1073/pnas.0404206101
– ident: R15
  doi: 10.1111/j.1558-5646.1985.tb00420.x
– ident: R4
  doi: 10.1099/ijs.0.65373-0
– ident: R25
  doi: 10.1128/JB.01962-12
– ident: R16
  doi: 10.1093/bioinformatics/btx713
SSID ssj0014600
Score 2.3572469
Snippet Four strains, KC07070 T , KC07105, 11 025B-8C and 11 026B-8-C, were isolated from the oral cavity of cats in 2007 or 2011 in Japan. These strains were...
Four strains, KC07070 , KC07105, 11 025B-8C and 11 026B-8-C, were isolated from the oral cavity of cats in 2007 or 2011 in Japan. These strains were...
Four strains, KC07070T, KC07105, 11 025B-8C and 11 026B-8-C, were isolated from the oral cavity of cats in 2007 or 2011 in Japan. These strains were...
SourceID proquest
pubmed
crossref
SourceType Aggregation Database
Index Database
Enrichment Source
StartPage 3355
Title Capnocytophaga felis sp. nov. isolated from the feline oral cavity
URI https://www.ncbi.nlm.nih.gov/pubmed/32375938
https://www.proquest.com/docview/2399831012
Volume 70
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3db9MwELdgCIkXNL7LABkJiYcoJU6cr0eYNgbaxksr9S26ODYqrEm1tJO2v567OE1S0UmDlyhybSe9--lytu9-x9gHlaNTbYRw0fh7Lho8cMEPtauMMX4C0vg5ZSOfnUcnU_l9Fs76E90mu2SVj9XNzryS_9EqtqFeKUv2HzTbTYoNeI_6xStqGK930vEhLMtKXRM3APwEx-iLee3Uy7FTVldjZ44PB3IouxQS6lBSJSciBQGqGjH0Tbc3BweUEgO2Z9pm11ftn6KAu8W8Z3LqD5hu1rYaNgWa2kAvap_il7fNQKsu1_3p_2LdFDtyzqCGssq7X74toPptB1Q00XCHwvf6eMDWqMoockOv3bTUO9paS2xLiLSICwdmNQgsl-9f9h79W1TS_FetF2OK0ZMi3kGsff4jO56enmaTo9nkPnvg44qCil18nXXRQPg6TbZS91odv2f6aXv2bf_llkVJ45xM9tnjdlXBP1uIPGH3dPmUPbR1Rq-fsS_bQOENUDgChRNQ-AYonIDCESjcAoUTULgFynM2PT6aHJ64bfEMVwWRWLlGSN_keQEgjG-kCk2UxqJIgwK0ELFCNzlJ0NhDDOhl55AYL9YiSaGIjIx1GLxge2VV6leMKxXFhZQ4UiYyNB66kDl28woh49BAMGLuRiSZapnlqcDJRWYjHNKsEWFGRLQkwhH72PVfWk6VW3u-30g4Q7NHZ1lQ6mpdZ5SSTTXyhD9iL63ou7kCP4jDNEhe32H0AXvUQ_YN21tdrvVbdDNX-bsGIH8AquKB4g
linkProvider Geneva Foundation for Medical Education and Research
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Capnocytophaga+felis+sp.+nov.+isolated+from+the+feline+oral+cavity&rft.jtitle=International+journal+of+systematic+and+evolutionary+microbiology&rft.au=Suzuki%2C+Michio&rft.au=Umeda%2C+Kaoru&rft.au=Kimura%2C+Masanobu&rft.au=Imaoka%2C+Koichi&rft.date=2020-05-01&rft.issn=1466-5034&rft.eissn=1466-5034&rft.volume=70&rft.issue=5&rft.spage=3355&rft_id=info:doi/10.1099%2Fijsem.0.004176&rft.externalDBID=NO_FULL_TEXT
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1466-5026&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1466-5026&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1466-5026&client=summon