Reduction of organic nitrates catalysed by xanthine oxidoreductase under anaerobic conditions

• Published in: Biochimica et biophysica acta Vol. 1527; no. 1; pp. 81 - 87
• Main Authors: Doel, Justin J, Godber, Ben L.J, Eisenthal, Robert, Harrison, Roger
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 02.07.2001
• Subjects: Anaerobiosis - physiology; Animals; Catalysis; Cattle; Flavin-Adenine Dinucleotide - metabolism; Glyceryl trinitrate; Molybdenum - metabolism; Nitrates - metabolism; Nitric oxide; Nitrites - analysis; Oxidation-Reduction; Xanthine - metabolism; Xanthine dehydrogenase; Xanthine Dehydrogenase - metabolism; Xanthine oxidase; Xanthine Oxidase - metabolism; IS-2-MN, isosorbide 2-mononitrate; Glyceryl trinitrate; XDH, xanthine dehydrogenase; Xanthine dehydrogenase; XO, xanthine oxidase; IS-5-MN, isosorbide 5-mononitrate; AFR, activity to flavin ratio; XOR, xanthine oxidoreductase; Nitric oxide; GTN, glyceryl trinitrate; Bicine, N, N-bis(2-hydroxyethyl)glycine; ISDN, isosorbide dinitrate; IDP, iodonium diphenyl; Xanthine oxidase

Xanthine oxidoreductase catalyses the anaerobic reduction of glyceryl trinitrate (GTN), isosorbide dinitrate and isosorbide mononitrate to inorganic nitrite using xanthine or NADH as reducing substrates. Reduction rates are much faster with xanthine as reducing substrate than with NADH. In the prese...