2-Benzyloxybenzaldehyde inhibits formyl-methionyl-leucyl-phenylalanine stimulation of phospholipase D activation in rat neutrophils

• Published in: Biochimica et biophysica acta Vol. 1573; no. 1; pp. 26 - 32
• Main Authors: Wang, Jih-Pyang, Chang, Ling-Chu, Hsu, Mei-Feng, Huang, Li-Jiau, Kuo, Sheng-Chu
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 10.10.2002
• Subjects: ADP-ribosylation factor; ADP-Ribosylation Factors - metabolism; Animals; Benzaldehydes - pharmacology; Calcium - metabolism; Cell Membrane - drug effects; Cell Membrane - metabolism; Dose-Response Relationship, Drug; Enzyme Activation - drug effects; Enzyme Inhibitors - pharmacology; Glycerophospholipids - metabolism; Intracellular free Ca 2; N-Formylmethionine Leucyl-Phenylalanine - antagonists & inhibitors; N-Formylmethionine Leucyl-Phenylalanine - pharmacology; Neutrophil; Neutrophils - drug effects; Neutrophils - metabolism; Phosphatidic Acids - metabolism; Phospholipase D; Phospholipase D - antagonists & inhibitors; Phospholipase D - metabolism; Protein tyrosine phosphorylation; Rats; Rats, Sprague-Dawley; Rho A; rhoA GTP-Binding Protein - metabolism; ADP-ribosylation factor; Neutrophil; Protein tyrosine phosphorylation; Intracellular free Ca 2; Phospholipase D; Rho A

2-Benzyloxybenzaldehyde (CCY1a) inhibited the formyl-methionyl-leucyl-phenylalanine (fMLP)-stimulated phospholipase D (PLD)-mediated products, phosphatidic acid (PA) and phosphatidylethanol (PEt) formation in rat neutrophils in a concentration-dependent manner with IC 50 values of 15.8±2.5 and 13.9±...