Live analysis of lagging chromosomes during anaphase and their effect on spindle elongation rate in fission yeast

• Published in: Journal of cell science Vol. 113 Pt 23; no. 23; pp. 4177 - 4191
• Main Authors: Pidoux, A L, Uzawa, S, Perry, P E, Cande, W Z, Allshire, R C
• Format: Journal Article
• Language: English
• Published: England 01.12.2000
• Subjects: Anaphase - physiology; Cell Nucleus - physiology; Chromosome Segregation - genetics; Chromosomes, Fungal - physiology; Fungal Proteins - genetics; Gene Expression Regulation, Fungal; Genes, Reporter; Green Fluorescent Proteins; Indicators and Reagents - metabolism; Kinetochores - physiology; Luminescent Proteins - genetics; Meiosis - physiology; Mitosis - physiology; Mutagenesis - physiology; Saccharomyces cerevisiae Proteins; Schizosaccharomyces - cytology; Schizosaccharomyces - genetics; Schizosaccharomyces pombe; Spindle Apparatus - genetics; Transcription Factors - genetics

The fission yeast Schizosaccharomyces pombe is widely used as a model system for studies of the cell cycle and chromosome biology. To enhance these studies we have fused GFP to the chromodomain protein Swi6p, thus allowing nuclear and chromosome behaviour to be followed in living cells using time-la...