A serum fucolectin isolated and characterized from sea bass Dicentrarchus labrax

• Published in: Biochimica et biophysica acta Vol. 1528; no. 2; pp. 196 - 202
• Main Authors: Cammarata, Matteo, Vazzana, Mirella, Chinnici, Cinzia, Parrinello, Nicolò
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 03.10.2001
• Subjects: Animals; Bass - blood; Bass - metabolism; Carbohydrates - pharmacology; Centrifugation; Dicentrarchus labrax; Electrophoresis, Polyacrylamide Gel; Fish; Fucolectin; Hemagglutination Tests; Hemagglutinin; Immunoblotting; Lectins - blood; Lectins - chemistry; Lectins - isolation & purification; Serum; Fish; Serum; Dicentrarchus labrax; Hemagglutinin; Fucolectin

A lectin specific for fucose and galactose was isolated by affinity chromatography on Sepharose CL-6B from the serum of Dicentrarchus labrax. The hemagglutinating activity against rabbit erythrocytes was calcium-independent, and reached its maximum at 37°C. Two protein components were found in the h...