Maribacter cobaltidurans sp. nov., a heavy-metal-tolerant bacterium isolated from deep-sea sediment
A Gram-stain-negative, aerobic, non-motile and rod-shaped bacterium, designated B1 , was isolated from deep-sea sediment collected from the South Atlantic Ocean. Cells of strain B1 grew in medium containing 0.5-6.0 % (w/v) NaCl (optimum 1.0-2.0 %), at pH 5.5-7.5 (optimum pH 7.0) and at 15-37 °C (opt...
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| Published in | International journal of systematic and evolutionary microbiology Vol. 67; no. 12; pp. 5261 - 5267 |
|---|---|
| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
| Published |
England
01.12.2017
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| Subjects | |
| Online Access | Get full text |
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| Abstract | A Gram-stain-negative, aerobic, non-motile and rod-shaped bacterium, designated B1
, was isolated from deep-sea sediment collected from the South Atlantic Ocean. Cells of strain B1
grew in medium containing 0.5-6.0 % (w/v) NaCl (optimum 1.0-2.0 %), at pH 5.5-7.5 (optimum pH 7.0) and at 15-37 °C (optimum 30 °C). Aesculin, starch and Tweens 20, 40 and 60 were hydrolysed. The strain was able to grow in medium containing a high concentration of Co
(10 mM). The 16S rRNA gene sequence of strain B1
shared high similarity with respect to Maribacter orientalis DSM 16471
(97.7 %), and exhibited less than 97.0 % sequence similarity with the type strains of other species with validly published names. Phylogenetic analyses revealed that strain B1
fell within the cluster comprising Maribacter species and formed an independent lineage. The average nucleotide identity and in silico DNA-DNA hybridization values between strain B1
and M. orientalis DSM 16471
were 72.0 % and 17.6 %, respectively. Strain B1
contained menaquinone 6 (MK-6) as the sole isoprenoid quinone and iso-C17 : 0 3-OH, iso-C15 : 0 and iso-C15 : 1 G as the predominant cellular fatty acids. The major polar lipids were phosphatidylethanolamine, one unidentified aminolipid and two unidentified lipids. The DNA G+C content was 39.7 mol%. According to the phylogenetic, chemotaxonomic and phenotypic data, B1
represents a novel species of the genus Maribacter, for which the name Maribacter cobaltidurans sp. nov. is proposed. The type strain is B1
(=CGMCC 1.15508
=KCTC 52882
=MCCC 1K03318
). |
|---|---|
| AbstractList | A Gram-stain-negative, aerobic, non-motile and rod-shaped bacterium, designated B1
, was isolated from deep-sea sediment collected from the South Atlantic Ocean. Cells of strain B1
grew in medium containing 0.5-6.0 % (w/v) NaCl (optimum 1.0-2.0 %), at pH 5.5-7.5 (optimum pH 7.0) and at 15-37 °C (optimum 30 °C). Aesculin, starch and Tweens 20, 40 and 60 were hydrolysed. The strain was able to grow in medium containing a high concentration of Co
(10 mM). The 16S rRNA gene sequence of strain B1
shared high similarity with respect to Maribacter orientalis DSM 16471
(97.7 %), and exhibited less than 97.0 % sequence similarity with the type strains of other species with validly published names. Phylogenetic analyses revealed that strain B1
fell within the cluster comprising Maribacter species and formed an independent lineage. The average nucleotide identity and in silico DNA-DNA hybridization values between strain B1
and M. orientalis DSM 16471
were 72.0 % and 17.6 %, respectively. Strain B1
contained menaquinone 6 (MK-6) as the sole isoprenoid quinone and iso-C17 : 0 3-OH, iso-C15 : 0 and iso-C15 : 1 G as the predominant cellular fatty acids. The major polar lipids were phosphatidylethanolamine, one unidentified aminolipid and two unidentified lipids. The DNA G+C content was 39.7 mol%. According to the phylogenetic, chemotaxonomic and phenotypic data, B1
represents a novel species of the genus Maribacter, for which the name Maribacter cobaltidurans sp. nov. is proposed. The type strain is B1
(=CGMCC 1.15508
=KCTC 52882
=MCCC 1K03318
). A Gram-stain-negative, aerobic, non-motile and rod-shaped bacterium, designated B1T, was isolated from deep-sea sediment collected from the South Atlantic Ocean. Cells of strain B1T grew in medium containing 0.5-6.0 % (w/v) NaCl (optimum 1.0-2.0 %), at pH 5.5-7.5 (optimum pH 7.0) and at 15-37 °C (optimum 30 °C). Aesculin, starch and Tweens 20, 40 and 60 were hydrolysed. The strain was able to grow in medium containing a high concentration of Co2+ (10 mM). The 16S rRNA gene sequence of strain B1T shared high similarity with respect to Maribacter orientalis DSM 16471T (97.7 %), and exhibited less than 97.0 % sequence similarity with the type strains of other species with validly published names. Phylogenetic analyses revealed that strain B1T fell within the cluster comprising Maribacter species and formed an independent lineage. The average nucleotide identity and in silico DNA-DNA hybridization values between strain B1T and M. orientalis DSM 16471T were 72.0 % and 17.6 %, respectively. Strain B1T contained menaquinone 6 (MK-6) as the sole isoprenoid quinone and iso-C17 : 0 3-OH, iso-C15 : 0 and iso-C15 : 1 G as the predominant cellular fatty acids. The major polar lipids were phosphatidylethanolamine, one unidentified aminolipid and two unidentified lipids. The DNA G+C content was 39.7 mol%. According to the phylogenetic, chemotaxonomic and phenotypic data, B1T represents a novel species of the genus Maribacter, for which the name Maribacter cobaltidurans sp. nov. is proposed. The type strain is B1T (=CGMCC 1.15508T=KCTC 52882T=MCCC 1K03318T).A Gram-stain-negative, aerobic, non-motile and rod-shaped bacterium, designated B1T, was isolated from deep-sea sediment collected from the South Atlantic Ocean. Cells of strain B1T grew in medium containing 0.5-6.0 % (w/v) NaCl (optimum 1.0-2.0 %), at pH 5.5-7.5 (optimum pH 7.0) and at 15-37 °C (optimum 30 °C). Aesculin, starch and Tweens 20, 40 and 60 were hydrolysed. The strain was able to grow in medium containing a high concentration of Co2+ (10 mM). The 16S rRNA gene sequence of strain B1T shared high similarity with respect to Maribacter orientalis DSM 16471T (97.7 %), and exhibited less than 97.0 % sequence similarity with the type strains of other species with validly published names. Phylogenetic analyses revealed that strain B1T fell within the cluster comprising Maribacter species and formed an independent lineage. The average nucleotide identity and in silico DNA-DNA hybridization values between strain B1T and M. orientalis DSM 16471T were 72.0 % and 17.6 %, respectively. Strain B1T contained menaquinone 6 (MK-6) as the sole isoprenoid quinone and iso-C17 : 0 3-OH, iso-C15 : 0 and iso-C15 : 1 G as the predominant cellular fatty acids. The major polar lipids were phosphatidylethanolamine, one unidentified aminolipid and two unidentified lipids. The DNA G+C content was 39.7 mol%. According to the phylogenetic, chemotaxonomic and phenotypic data, B1T represents a novel species of the genus Maribacter, for which the name Maribacter cobaltidurans sp. nov. is proposed. The type strain is B1T (=CGMCC 1.15508T=KCTC 52882T=MCCC 1K03318T). |
| Author | Xamxidin, Maripat Wang, Chun-Sheng Fang, Chen Xu, Xue-Wei Wu, Yue-Hong |
| Author_xml | – sequence: 1 givenname: Chen surname: Fang fullname: Fang, Chen organization: Key Laboratory of Marine Ecosystem and Biogeochemistry, Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, PR China – sequence: 2 givenname: Yue-Hong surname: Wu fullname: Wu, Yue-Hong organization: Key Laboratory of Marine Ecosystem and Biogeochemistry, Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, PR China – sequence: 3 givenname: Maripat surname: Xamxidin fullname: Xamxidin, Maripat organization: Key Laboratory of Marine Ecosystem and Biogeochemistry, Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, PR China – sequence: 4 givenname: Chun-Sheng surname: Wang fullname: Wang, Chun-Sheng organization: Key Laboratory of Marine Ecosystem and Biogeochemistry, Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, PR China – sequence: 5 givenname: Xue-Wei surname: Xu fullname: Xu, Xue-Wei organization: Key Laboratory of Marine Ecosystem and Biogeochemistry, Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, PR China |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/29087269$$D View this record in MEDLINE/PubMed |
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| CitedBy_id | crossref_primary_10_1007_s00203_022_02900_2 crossref_primary_10_1111_1462_2920_15650 crossref_primary_10_1007_s00203_021_02696_7 crossref_primary_10_1016_j_margen_2017_12_005 crossref_primary_10_3389_fmars_2023_1248754 crossref_primary_10_1016_j_pocean_2018_09_015 crossref_primary_10_1099_ijsem_0_005218 crossref_primary_10_1099_ijsem_0_004678 crossref_primary_10_1099_ijsem_0_002843 crossref_primary_10_1111_1751_7915_14479 crossref_primary_10_1128_spectrum_02464_22 crossref_primary_10_1099_ijsem_0_005236 crossref_primary_10_1099_ijsem_0_005786 crossref_primary_10_1007_s10482_023_01844_x crossref_primary_10_1007_s10482_023_01888_z crossref_primary_10_1099_ijsem_0_003377 crossref_primary_10_1099_ijsem_0_003011 crossref_primary_10_1007_s10482_024_01950_4 |
| Cites_doi | 10.1099/ijs.0.006056-0 10.1099/00207713-52-3-1049 10.1099/ijs.0.023325-0 10.1099/ijs.0.039628-0 10.1007/BF01734359 10.1099/ijs.0.65469-0 10.1093/molbev/mst197 10.1099/ijs.0.052951-0 10.1099/ijs.0.65022-0 10.1128/JB.85.5.1183-1184.1963 10.1093/nar/gkm864 10.1099/ijsem.0.001341 10.1099/ijs.0.65549-0 10.1099/ijs.0.000224 10.1099/ijsem.0.000526 10.1099/ijsem.0.001755 10.1099/ijs.0.064774-0 10.1099/ijs.0.012286-0 10.1099/ijsem.0.000942 10.1099/ijs.0.02520-0 10.1099/ijs.0.02849-0 10.1073/pnas.0906412106 10.1093/nar/22.22.4673 10.1099/ijs.0.040915-0 10.1099/00207713-37-4-463 10.2307/2412116 10.1016/j.syapm.2008.07.001 10.1099/ijs.0.65181-0 10.1007/s10482-014-0243-z 10.1099/ijs.0.050054-0 10.1007/BF01731581 10.1099/ijs.0.000379 10.1093/nar/gkh293 10.1099/ijs.0.63777-0 10.1007/s002530051457 10.1007/s10534-011-9473-y 10.1093/nar/gkm160 |
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| Keywords | Flavobacteria Maribacter heavy-metal-tolerant |
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| References | Bernardet (R39) 2010; vol. 4 Leifson (R32) 1963; 85 R41 R40 R21 Farmer III (R30) 1854; vol. 2 R20 R42 R23 R22 R25 R27 R26 R29 R1 R2 R3 Moore (R38) 1987; 37 R4 R5 R6 R7 Tindall (R33) 2007 R8 R9 Cai (R28) 2001 Saitou (R24) 1987; 4 R10 R31 R12 R34 R11 R14 R36 R13 R35 R16 R15 R37 R18 R17 R19 |
| References_xml | – ident: R10 doi: 10.1099/ijs.0.006056-0 – ident: R40 doi: 10.1099/00207713-52-3-1049 – ident: R35 doi: 10.1099/ijs.0.023325-0 – ident: R4 doi: 10.1099/ijs.0.039628-0 – ident: R26 doi: 10.1007/BF01734359 – ident: R2 doi: 10.1099/ijs.0.65469-0 – start-page: 330 volume-title: Methods for General and Molecular Microbiology year: 2007 ident: R33 article-title: Phenotypic characterization and the principles of comparative systematics – ident: R23 doi: 10.1093/molbev/mst197 – ident: R29 doi: 10.1099/ijs.0.052951-0 – ident: R16 doi: 10.1099/ijs.0.65022-0 – volume: 85 start-page: 1183 year: 1963 ident: R32 article-title: Determination of carbohydrate metabolism of marine bacteria publication-title: J Bacteriol doi: 10.1128/JB.85.5.1183-1184.1963 – ident: R21 doi: 10.1093/nar/gkm864 – ident: R41 doi: 10.1099/ijsem.0.001341 – ident: R11 doi: 10.1099/ijs.0.65549-0 – ident: R7 doi: 10.1099/ijs.0.000224 – volume-title: Determinative Manual for Routine Bacteriology year: 2001 ident: R28 – ident: R36 doi: 10.1099/ijsem.0.000526 – ident: R18 doi: 10.1099/ijsem.0.001755 – ident: R6 doi: 10.1099/ijs.0.064774-0 – volume: vol. 4 start-page: 105 volume-title: Bergey's Manual of Systematic Bacteriology year: 2010 ident: R39 article-title: Flavobacteriales ord. nov – ident: R3 doi: 10.1099/ijs.0.012286-0 – ident: R12 doi: 10.1099/ijsem.0.000942 – volume: 4 start-page: 406 year: 1987 ident: R24 article-title: The neighbor-joining method: a new method for reconstructing phylogenetic trees publication-title: Mol Biol Evol – ident: R31 doi: 10.1099/ijs.0.02520-0 – ident: R1 doi: 10.1099/ijs.0.02849-0 – ident: R37 doi: 10.1073/pnas.0906412106 – ident: R22 doi: 10.1093/nar/22.22.4673 – ident: R34 doi: 10.1099/ijs.0.040915-0 – volume: 37 start-page: 463 year: 1987 ident: R38 article-title: Report of the ad hoc committee on reconciliation of approaches to bacterial systematics publication-title: Int. J. Syst. Bacteriol doi: 10.1099/00207713-37-4-463 – ident: R25 doi: 10.2307/2412116 – volume: vol. 2 start-page: 494 volume-title: Bergey's Manual of Systematic Bacteriology year: 1854 ident: R30 article-title: Genus I. Vibrio Pacini 1854, 411AL – ident: R20 doi: 10.1016/j.syapm.2008.07.001 – ident: R9 doi: 10.1099/ijs.0.65181-0 – ident: R13 doi: 10.1007/s10482-014-0243-z – ident: R5 doi: 10.1099/ijs.0.050054-0 – ident: R27 doi: 10.1007/BF01731581 – ident: R42 doi: 10.1099/ijs.0.000379 – ident: R19 doi: 10.1093/nar/gkh293 – ident: R8 doi: 10.1099/ijs.0.63777-0 – ident: R14 doi: 10.1007/s002530051457 – ident: R15 doi: 10.1007/s10534-011-9473-y – ident: R17 doi: 10.1093/nar/gkm160 |
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| Snippet | A Gram-stain-negative, aerobic, non-motile and rod-shaped bacterium, designated B1
, was isolated from deep-sea sediment collected from the South Atlantic... A Gram-stain-negative, aerobic, non-motile and rod-shaped bacterium, designated B1T, was isolated from deep-sea sediment collected from the South Atlantic... |
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| Title | Maribacter cobaltidurans sp. nov., a heavy-metal-tolerant bacterium isolated from deep-sea sediment |
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