Synthesis of the milk oligosaccharide 2′-fucosyllactose using recombinant bacterial enzymes

• Published in: Carbohydrate research Vol. 334; no. 2; pp. 97 - 103
• Main Authors: Albermann, Christoph, Piepersberg, Wolfgang, Wehmeier, Udo F
• Format: Journal Article
• Language: English
• Published: OXFORD Elsevier Ltd 23.08.2001; Elsevier
• Subjects: 2′-Fucosyllactose; Animals; Bacterial α-(1→2)-fucosyltransferase; Biochemistry & Molecular Biology; Chemistry; Chemistry, Applied; Chemistry, Organic; Enzymatic synthesis; Escherichia coli - enzymology; Fucosyltransferases - metabolism; GDP- l-fucose; Guanosine Diphosphate Fucose - biosynthesis; Guanosine Diphosphate Fucose - metabolism; Helicobacter pylori - genetics; Helicobacter pylori - metabolism; Helicobacter pylori, Escherichia coli; Hydro-Lyases - metabolism; Lactose - metabolism; Life Sciences & Biomedicine; Milk - chemistry; Physical Sciences; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Science & Technology; Trisaccharides - biosynthesis; Trisaccharides - chemistry; Trisaccharides - isolation & purification; Enzymatic synthesis; Bacterial α-(1→2)-fucosyltransferase; Helicobacter pylori, Escherichia coli; 2′-Fucosyllactose; GDP- l-fucose; COLANIC ACID; 2 '-fucosyllactose; bacterial alpha-(1 -> 2)-fucosyltransferase; GDP-FUCOSE; Escherichia coli; enzymatic synthesis; Helicobacter pylori; GENE-CLUSTER; CLONING; GDP-L-fucose; BIOSYNTHESIS; ESCHERICHIA-COLI K-12; MANNOSE PYROPHOSPHORYLASE; HELICOBACTER-PYLORI; EXPRESSION; TRANSFERASE

The enzymatic synthesis of GDP-β- l-fucose and its enzymatic transfer reaction using recombinant enzymes from bacterial sources was examined. The GDP- d-mannose 4,6-dehydratase and the GDP-4-keto-6-deoxy- d-mannose 3,5-epimerase-4-reductase from Escherichia coli K-12, respectively, were used to cata...