Non-Invasive Morphological Characterization of Rice Leaf Bulliform and Aerenchyma Cellular Regions Using Low Coherence Interferometry

• Published in: Applied sciences Vol. 9; no. 10; p. 2104
• Main Authors: Kim, Hyeree, Du, XiaoXuan, Kim, Sungwook, Kim, Pilun, Wijesinghe, Ruchire Eranga, Yun, Byoung-Ju, Kim, Kyung-Min, Jeon, Mansik, Kim, Jeehyun
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01.05.2019
• Subjects: Aerenchyma cellular region; Agricultural products; Agriculture; biological tomographic imaging; Bulliform cellular region; Datasets; Dentistry; Deoxyribonucleic acid; DNA; Global economy; High resolution; Impact analysis; Inspection; Interferometry; Low coherence interferometry; Medical imaging; morphological; Morphology; Mutation; NMR; Nondestructive testing; Nuclear magnetic resonance; Ophthalmology; Optical Coherence Tomography; Otolaryngology; Plant diseases; Real time; Rice; rice leaf; Spatial resolution; swept source optical coherence tomography (SS-OCT)
• DOI: 10.3390/app9102104
• ISSN: 2076-3417

Non-invasive investigation of rice leaf specimens to characterize the morphological formation and particular structural information that is beneficial for agricultural perspective was demonstrated using a low coherence interferometric method called swept source optical coherence tomography (SS-OCT). The acquired results non-invasively revealed morphological properties of rice leaf, such as bulliform cells; aerenchyma, parenchyma, and collenchyma layer; and vascular bundle. Beside aforementioned morphologic characteristics, several leaf characteristics associated with cytological mechanisms of leaf rolling (leaf inclination) were examined for the pre-identification of inevitable necrosis and atrophy of leaf tissues by evaluating acute angle information, such as angular characteristics of the external bi-directional angles between the lower epidermis layer and lower mid-vein, and internal angle of lower mid-vein. To further assist the pre-identification, acquired cross-sections were employed to enumerate the small veins of each leaf specimen. Since mutants enlarge leaf angles due to increased cell division in the adaxial epidermis, healthy and abnormal leaf specimens were morphologically and quantitatively compared. Therefore, the results of the method can be used in agriculture, and SS-OCT shows potential as a rigorous investigation method for selecting mutant infected rice leaf specimens rapidly and non-destructively compared to destructive and time consuming gold-standard methods with a lack of precision.