Importance of glycosidases in mammalian glycoprotein biosynthesis

• Published in: BBA - General Subjects Vol. 1473; no. 1; pp. 96 - 107
• Main Author: Herscovics, Annette
• Format: Book Review Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 06.12.1999
• Subjects: alpha-Glucosidases - metabolism; alpha-Mannosidase; Animals; Endo α-mannosidase; Endoplasmic Reticulum - enzymology; Glycoprotein folding; Glycoproteins - biosynthesis; Glycoside Hydrolases - metabolism; Golgi Apparatus - enzymology; Humans; Mannosidases - metabolism; N-Glycan processing; Quality control; α-Glucosidase; α-Mannosidase; N-Glycan processing; Glycoprotein folding; SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis; α-Glucosidase; RT-PCR, reverse transcriptase-polymerase chain reaction; α-Mannosidase; Endo α-mannosidase; Quality control; ER, endoplasmic reticulum

Processing glycosidases play an important role in N-glycan biosynthesis in mammalian cells by trimming Glc 3Man 9GlcNAc 2 and thus providing the substrates for the formation of complex and hybrid structures by Golgi glycosyltransferases. Processing glycosidases also play a role in the folding of new...