Streptobacillus ratti sp. nov., isolated from a black rat (Rattus rattus)

• Published in: International journal of systematic and evolutionary microbiology Vol. 66; no. 4; pp. 1620 - 1626
• Main Authors: Eisenberg, Tobias, Imaoka, Koichi, Kimura, Masanobu, Glaeser, Stefanie P., Ewers, Christa, Semmler, Torsten, Rau, Jörg, Nicklas, Werner, Tanikawa, Tsutomu, Kämpfer, Peter
• Format: Journal Article
• Language: English
• Published: England 01.04.2016
• Subjects: Animals; Bacterial Typing Techniques; Base Composition; DNA, Bacterial - genetics; Fatty Acids - chemistry; Genes, Bacterial; Japan; Molecular Sequence Data; Mouth - microbiology; Nucleic Acid Hybridization; Phylogeny; Rats - microbiology; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Streptobacillus - classification; Streptobacillus - genetics; Streptobacillus - isolation & purification; Japan
• ISSN: 1466-5026; 1466-5034; 1466-5034
• DOI: 10.1099/ijsem.0.000869

An indole-, oxidase- and catalase-negative, non-motile bacterium, strain OGS16T, was isolated from an oral swab of a feral black rat (Rattus rattus) in 2007 in Japan. It stained Gram-negative and had pleomorphic, rod-shaped, non-spore-forming cells. Based on 16S rRNA gene sequence analyses, strain OGS16T was assigned to the genus Streptobacillus, with 16S rRNA gene sequence similarities of 99.3, 99.0, 98.6 and 95.5% to the type strains of Streptobacillus moniliformis, Streptobacillus notomytis, Streptobacillus felis and Streptobacillus hongkongensis, respectively. Strain OGS16T could also be differentiated clearly from other species of the genus Streptobacillus by rpoB, groEL and recA nucleotide and deduced amino acid sequence analysis. DNA-DNA relatedness as obtained by average nucleotide identity was 89.10% between strain OGS16T and Streptobacillus moniliformis DSM 12112T. Chemotaxonomic and physiological data for strain OGS16T were congruent with results for other closely related members of the family Leptotrichiaceae, represented by highly similar enzyme profiles and fatty acid patterns. MALDI-TOF MS analysis also proved suitable in discriminating strain OGS16T unequivocally from all currently described taxa of the genus Streptobacillus. On the basis of these data, we propose the novel species Streptobacillus ratti sp. nov., with the type strain OGS16T (=JCM 31098T=DSM 101843T). The G+C content of the DNA of the type strain is 25.9 mol% and the genome size is 1.50 Mbp.