In vitro maturation of bitch oocytes from advanced preantral follicles in synthetic oviduct fluid medium: serum is not essential

• Published in: Theriogenology Vol. 58; no. 9; pp. 1689 - 1703
• Main Authors: Bolamba, Digbo, Russ, Kara D, Olson, Mary A, Sandler, Jody L, Durrant, Barbara S
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.12.2002
• Subjects: Animals; Body Fluids; Cells, Cultured; Culture Media; Culture Media, Serum-Free; Dogs; Fallopian Tubes - metabolism; Female; Meiosis; Metaphase; Oocyte; Oocytes - physiology; Oocytes - ultrastructure; Ovarian Follicle - cytology; Preantral follicle; Reproductive technology; Serum Albumin, Bovine; Meiosis; Preantral follicle; Oocyte; Reproductive technology

The ability of oocytes from preantral follicles to mature in vitro was assessed using a synthetic oviduct fluid (SOF) medium. Advanced preantral follicles (approximately 210μm diameter) were isolated from the ovaries of domestic bitches and assigned to one of four treatment groups: (1) SOF (n=230);...