Biochemical characterization of two crotamine isoforms isolated by a single step RP-HPLC from Crotalus durissus terrificus (South American rattlesnake) venom and their action on insulin secretion by pancreatic islets

• Published in: Biochimica et biophysica acta Vol. 1474; no. 1; pp. 56 - 60
• Main Authors: Toyama, Marcos H., Carneiro, Everardo M., Marangoni, Sergio, Barbosa, Rosicler L., Corso, Gaetano, Boschero, Antonio C.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 06.03.2000
• Subjects: Animals; Chromatography, Gel; Chromatography, High Pressure Liquid; Crotalid Venoms - chemistry; Crotalid Venoms - isolation & purification; Crotalid Venoms - pharmacology; Crotalus durrisus terrificus; Crotamine; Insulin - metabolism; Insulin Secretion; Islets of Langerhans - drug effects; Islets of Langerhans - metabolism; Mass Spectrometry; Molecular Structure; Molecular Weight; Na + channel; Pancreatic β-cell; Protein Isoforms - chemistry; Rats; Sequence Homology; South America; Venom; South America; Crotalus durrisus terrificus; Pancreatic β-cell; Venom; Crotamine; Na + channel; Insulin secretion

Crotamine, a neurotoxin present in the venom of the South American rattlesnake Crotalus durrisus terrificus exists as several polymorphic variants, as demonstrated by recombinant DNA technology (Smith and Schmidt, Toxicon 28 (1990) 575–585). We have isolated native crotamine by chromatography on Sep...