Development and characterization of simulant pancreatic islets

Insulin is stored in pancreatic islets as a zinc–insulin complex, and stimulating the islets results in the release of insulin and zinc. Simulant pancreatic islet beads have been developed using agarose beads (50–250 μm diameter) derivatized with iminodiacetic acid that have been loaded with zinc. A...

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Published inAnalytical biochemistry Vol. 314; no. 1; pp. 38 - 45
Main Authors Maghasi, Anne T., Schlueter, Kevin T., Halsall, H.Brian, Heineman, William R., Rodriguez Rilo, Horacio L.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.03.2003
Subjects
Animals
Anodic stripping voltammetry
Dogs
Electrochemistry
Hydrochloric Acid - pharmacology
Insulin - metabolism
Insulin Secretion
Islets of Langerhans - drug effects
Islets of Langerhans - metabolism
Microspheres
Pancreatic islets
Rats
Sepharose - metabolism
Sepharose - pharmacology
Simulant beads
Staining and Labeling
Zinc
Zinc - metabolism
Simulant beads
Pancreatic islets
Anodic stripping voltammetry
Zinc
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Abstract Insulin is stored in pancreatic islets as a zinc–insulin complex, and stimulating the islets results in the release of insulin and zinc. Simulant pancreatic islet beads have been developed using agarose beads (50–250 μm diameter) derivatized with iminodiacetic acid that have been loaded with zinc. A qualitative comparison of the simulant beads with pancreatic islets has been made by staining with dithizone and a zinc-binding fluorescent dye, TSQ. The binding capacity of simulant beads was determined to be 34 μmol Zn 2+/g of dried beads using anodic stripping voltammetry. Hydrochloric acid was used to release zinc from beads to mimic the secretion of insulin from pancreatic islets and a release profile was established. The simulant beads can be used to optimize the islet isolation process and reduce the use of real islets in method development.
AbstractList Insulin is stored in pancreatic islets as a zinc-insulin complex, and stimulating the islets results in the release of insulin and zinc. Simulant pancreatic islet beads have been developed using agarose beads (50-250 micro m diameter) derivatized with iminodiacetic acid that have been loaded with zinc. A qualitative comparison of the simulant beads with pancreatic islets has been made by staining with dithizone and a zinc-binding fluorescent dye, TSQ. The binding capacity of simulant beads was determined to be 34 micro mol Zn(2+)/g of dried beads using anodic stripping voltammetry. Hydrochloric acid was used to release zinc from beads to mimic the secretion of insulin from pancreatic islets and a release profile was established. The simulant beads can be used to optimize the islet isolation process and reduce the use of real islets in method development.
Insulin is stored in pancreatic islets as a zinc–insulin complex, and stimulating the islets results in the release of insulin and zinc. Simulant pancreatic islet beads have been developed using agarose beads (50–250 μm diameter) derivatized with iminodiacetic acid that have been loaded with zinc. A qualitative comparison of the simulant beads with pancreatic islets has been made by staining with dithizone and a zinc-binding fluorescent dye, TSQ. The binding capacity of simulant beads was determined to be 34 μmol Zn 2+/g of dried beads using anodic stripping voltammetry. Hydrochloric acid was used to release zinc from beads to mimic the secretion of insulin from pancreatic islets and a release profile was established. The simulant beads can be used to optimize the islet isolation process and reduce the use of real islets in method development.
Author Rodriguez Rilo, Horacio L.
Schlueter, Kevin T.
Maghasi, Anne T.
Halsall, H.Brian
Heineman, William R.
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Cites_doi 10.1016/S0041-1345(01)02416-2
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Keywords Simulant beads
Pancreatic islets
Anodic stripping voltammetry
Zinc
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Snippet Insulin is stored in pancreatic islets as a zinc–insulin complex, and stimulating the islets results in the release of insulin and zinc. Simulant pancreatic...
Insulin is stored in pancreatic islets as a zinc-insulin complex, and stimulating the islets results in the release of insulin and zinc. Simulant pancreatic...
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SubjectTerms Animals
Anodic stripping voltammetry
Dogs
Electrochemistry
Hydrochloric Acid - pharmacology
Insulin - metabolism
Insulin Secretion
Islets of Langerhans - drug effects
Islets of Langerhans - metabolism
Microspheres
Pancreatic islets
Rats
Sepharose - metabolism
Sepharose - pharmacology
Simulant beads
Staining and Labeling
Zinc
Zinc - metabolism
Title Development and characterization of simulant pancreatic islets
URI https://dx.doi.org/10.1016/S0003-2697(02)00626-7
https://www.ncbi.nlm.nih.gov/pubmed/12633600
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