Immunological determination and size characterization of poly(ADP-ribose) synthesized in vitro and in vivo

• Published in: Biochimica et biophysica acta Vol. 1428; no. 2; pp. 137 - 146
• Main Authors: Affar, El Bachir, Duriez, Patrick J., Shah, Rashmi G., Winstall, Eric, Germain, Marc, Boucher, Claudia, Bourassa, Sylvie, Kirkland, James B., Poirier, Guy G.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 05.08.1999
• Subjects: Animals; Anti-poly(ADP-ribose) antibody; Antibodies - immunology; Antibody Specificity; Cell Line; Chemical Fractionation; DNA damage; Electrophoresis, Polyacrylamide Gel; Enzyme-Linked Immunosorbent Assay; Flow Cytometry; Immunoblotting; Immunohistochemistry; Methylnitronitrosoguanidine; Mice; N-Methyl- N′-nitro- N-nitrosoguanidine; Poly Adenosine Diphosphate Ribose - biosynthesis; Poly Adenosine Diphosphate Ribose - chemistry; Poly Adenosine Diphosphate Ribose - immunology; Poly(ADP-ribose) polymerase; Poly(ADP-ribose) size; N-Methyl- N′-nitro- N-nitrosoguanidine; DNA damage; Anti-poly(ADP-ribose) antibody; DHBB, dihydroxyboronyl Bio-Rex; Poly(ADP-ribose) polymerase; ABTS, 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid); DHQ, 1,5-dihydroxyisoquinoline; PARG, poly(ADP-ribose) glycohydrolase; MNNG, N-methyl- N′-nitro- N-nitrosoguanidine; XC, xylene cyanol; Poly(ADP-ribose) size; pADPr, poly(ADP-ribose); BPB, bromophenol blue; PARP, poly(ADP-ribose) polymerase

Poly(ADP-ribose) polymerase is a DNA break detecting enzyme playing a role in the surveillance of genome integrity. Poly(ADP-ribose) is synthesized rapidly and transiently from β-NAD in response to DNA damaging agents. In order to study the physiological significance of poly(ADP-ribose) metabolism,...