The influence of the Ustilago maydis REC1 gene on plasmid-chromosome recombination

• Published in: Current genetics Vol. 22; no. 6; p. 483
• Main Authors: Banks, G.R. (National Institute for Medical Research, London (United Kingdom). Genetics Div.), Kanuga, N, Ealing, J, Spanos, A, Holden, D.W
• Format: Journal Article
• Language: English
• Published: United States 01.12.1992
• Subjects: CHROMOSOME; CHROMOSOMES; Chromosomes, Fungal; CROMOSOMAS; DNA; Exodeoxyribonuclease V; Fungal Proteins - genetics; GENE; GENES; Genes, Fungal; Nucleic Acid Hybridization; Plasmid-chromosome recombination; PLASMIDE; PLASMIDIOS; PLASMIDS; REC1; RECOMBINACION; RECOMBINAISON; RECOMBINATION; Recombination, Genetic; Restriction Mapping; Transformation, Genetic; USTILAGO; Ustilago - genetics

The REC1 gene of U. maydis has an important but ill-defined role in DNA recombination and repair. We have examined its role in plasmid-chromosome recombination. Plasmid DNA was linearized at various locations with respect to the cloned U. maydis PYR3 gene and introduced into cells by transformation....