The influence of the Ustilago maydis REC1 gene on plasmid-chromosome recombination

The REC1 gene of U. maydis has an important but ill-defined role in DNA recombination and repair. We have examined its role in plasmid-chromosome recombination. Plasmid DNA was linearized at various locations with respect to the cloned U. maydis PYR3 gene and introduced into cells by transformation....

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Published inCurrent genetics Vol. 22; no. 6; p. 483
Main Authors Banks, G.R. (National Institute for Medical Research, London (United Kingdom). Genetics Div.), Kanuga, N, Ealing, J, Spanos, A, Holden, D.W
Format Journal Article
LanguageEnglish
Published United States 01.12.1992
Subjects
CHROMOSOME
CHROMOSOMES
Chromosomes, Fungal
CROMOSOMAS
DNA
Exodeoxyribonuclease V
Fungal Proteins - genetics
GENE
GENES
Genes, Fungal
Nucleic Acid Hybridization
Plasmid-chromosome recombination
PLASMIDE
PLASMIDIOS
PLASMIDS
REC1
RECOMBINACION
RECOMBINAISON
RECOMBINATION
Recombination, Genetic
Restriction Mapping
Transformation, Genetic
USTILAGO
Ustilago - genetics
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Summary:The REC1 gene of U. maydis has an important but ill-defined role in DNA recombination and repair. We have examined its role in plasmid-chromosome recombination. Plasmid DNA was linearized at various locations with respect to the cloned U. maydis PYR3 gene and introduced into cells by transformation. Chromosomal integration and repair by an homologous cross-over with plasmid containing a double-strand break or gap in the PYR3 gene was markedly reduced in the absence of the REC1 gene product. Homologous replacement of the chromosomal pyr3-1 allele by a single copy of wild-type sequences from plasmid cut outside PYR3 was not found in the absence of the REC1 product. Instead, novel transformants generated in its absence suggests that ligation plays a role in their generation.
Bibliography:F30
93B0566
ISSN:0172-8083
1432-0983
DOI:10.1007/BF00326414