Isozyme analysis of Kenyan phlebotomine sandflies (Diptera: Ppsychodidae) by isoelectric focusing (IEF) on pharmacia phast system

The isoenzyme profiles of nine sandfly species ( Phlebotomus duboscqi, P. elegonensi, P. martini, P. pedifer, Sergentomiya antennatus, S. bedfordi, S. garnhami, S. graingeri and S. schwetzi) from Kenya collected from the field and reared in the Medical Vectors Research Programme of the ICIPE were de...

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Published inBiochemical systematics and ecology Vol. 20; no. 7; pp. 593 - 596
Main Authors Mahamat, H., Hassanali, A., Morgan, H., Mutinaga, M.J., Mihok, S., Massamba, N.N.
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 01.01.1992
Subjects
chemotaxonomy
cluster analysis
Diptera
extracts
isozymes
Phlebotomus
Psychodidae
Sergentomyia
Kenya
Phlebotomus
Sergetomyia
Phlebotominae
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Summary:The isoenzyme profiles of nine sandfly species ( Phlebotomus duboscqi, P. elegonensi, P. martini, P. pedifer, Sergentomiya antennatus, S. bedfordi, S. garnhami, S. graingeri and S. schwetzi) from Kenya collected from the field and reared in the Medical Vectors Research Programme of the ICIPE were determined by isoelectric focusing (IEF) on mini ultra-thin polyacrylamide gels supplied by Pharmacia. Three of the nine isoenzymes screened (MDH, G-6-PD, PGM, ME, ICD, GAPDH, 6-PDG, HK and GPI), namely GPI, PGM and ME, gave banding patterns of taxonomic value. Cluster analysis of the simple matching coefficients gave two distinct groupings corresponding to previously described Sergentomyia and Phlebotomus respectively. However, S. garnhami, previously grouped in the genus Sergentomyia, was found to be associated with the genus Phlebotomus.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0305-1978
1873-2925
DOI:10.1016/0305-1978(92)90016-7