Location of O-acetyl groups in S-657 using the reductive-cleavage method

• Published in: Carbohydrate research Vol. 331; no. 3; pp. 265 - 270
• Main Authors: Diltz, Sandra, Zeller, Samuel G.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 12.04.2001
• Subjects: Acetylation; Carbohydrate Conformation; Carbohydrate Sequence; Chromatography, Gas; Hydrolysis; Methods; Methylation; Molecular Sequence Data; Molecular Structure; O-Acetyl groups; Oxidation-Reduction; Polysaccharide; Polysaccharides, Bacterial - chemistry; S-657; Sphingomonas - chemistry; Structural analysis; S-657; O-Acetyl groups; Polysaccharide; Structural analysis

A two-step procedure is described in this paper to identify the position of O-acetyl groups in S-657 polysaccharide. Reductive-cleavage experiments performed on the fully methylated (base-catalyzed) polysaccharide, followed by acetylation of anhydroalditols, identified individual sugar residues and...