High-Resolution Fluorespirometry to Assess Dynamic Changes in Mitochondrial Membrane Potential in Human Immune Cells

• Published in: Journal of visualized experiments no. 207
• Main Authors: Valencia, Ana P, Pharaoh, Gavin, Brandao, Arthur F, Marcinek, David J
• Format: Journal Article
• Language: English
• Published: United States 24.05.2024
• Subjects: Adenosine Diphosphate - metabolism; Adenosine Diphosphate - pharmacology; Fluorescent Dyes - chemistry; Humans; Leukocytes, Mononuclear - cytology; Leukocytes, Mononuclear - metabolism; Membrane Potential, Mitochondrial - physiology; Mitochondria - metabolism; Oxygen Consumption - physiology; Rhodamines - chemistry

Peripheral mononuclear cells (PBMCs) exhibit robust changes in mitochondrial respiratory capacity in response to health and disease. While these changes do not always reflect what occurs in other tissues, such as skeletal muscle, these cells are an accessible and valuable source of viable mitochondr...