The rate-limiting enzyme in phosphatidylcholine synthesis is associated with nuclear speckles under stress conditions

• Published in: Biochimica et biophysica acta Vol. 1801; no. 11; pp. 1184 - 1194
• Main Authors: Favale, Nicolás O., Fernández-Tome, María C., Pescio, Lucila G., Sterin-Speziale, Norma B.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.11.2010
• Subjects: Animals; Cell Line; Cell Nucleus - metabolism; Choline-Phosphate Cytidylyltransferase - chemistry; Choline-Phosphate Cytidylyltransferase - physiology; CTP:Phosphocholine Cytidylyltransferase; Cytoplasm - metabolism; Dogs; Endoplasmic Reticulum - metabolism; Enzymes - chemistry; Gene Silencing; Hypertonic stress; Lamin Type A - chemistry; Microscopy, Confocal - methods; Microscopy, Fluorescence - methods; Nuclear organization; Phosphatidylcholines - biosynthesis; Protein Transport; Speckle; Time Factors; NR; CCT; Nuclear organization; Speckle; Hypertonic stress; TOTO-3; CTP:Phosphocholine Cytidylyltransferase; ER; DIC

Phosphatidylcholine (PtdCho) is the most abundant phospholipid in eukaryotic membranes and its biosynthetic pathway is generally controlled by CTP:Phosphocholine Cytidylyltransferase (CCT), which is considered the rate-limiting enzyme. CCT is an amphitropic protein, whose enzymatic activity is commo...