The influence of N-glycosylation on biochemical properties of Amy1, an α-amylase from the yeast Cryptococcus flavus

• Published in: Carbohydrate research Vol. 344; no. 13; pp. 1682 - 1686
• Main Authors: de Barros, Manoel Cardoso, do Nascimento Silva, Roberto, Ramada, Marcelo Henrique Soller, Galdino, Alexsandro Sobreira, de Moraes, Lídia Maria Pepe, Torres, Fernando Araripe Gonçalves, Ulhoa, Cirano José
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 08.09.2009
• Subjects: alpha-Amylases - chemistry; alpha-Amylases - isolation & purification; alpha-Amylases - metabolism; Cell Proliferation - drug effects; Cryptococcus - cytology; Cryptococcus - enzymology; Cryptococcus flavus; Extracellular Space - drug effects; Extracellular Space - metabolism; Glycosylation - drug effects; Intracellular Space - drug effects; Intracellular Space - metabolism; Nitrogen - metabolism; Tunicamycin; Tunicamycin - pharmacology; α-Amylase, N-glycosylation; Cryptococcus flavus; Tunicamycin; α-Amylase, N-glycosylation

The yeast Cryptococcus flavus secretes a glycosylated α-amylase (Amy1) when grown in a starch-containing medium. The effects of N-glycosylation on secretion, enzyme activity, and stability of this glycoprotein were studied. Addition of tunicamycin (TM) to the medium at a concentration higher than 0....