Membrane bilayer properties of sphingomyelins with amide-linked 2- or 3-hydroxylated fatty acids

• Published in: Biochimica et biophysica acta Vol. 1808; no. 3; pp. 727 - 732
• Main Authors: Ekholm, Oscar, Jaikishan, Shishir, Lönnfors, Max, Nyholm, Thomas K.M., Slotte, J. Peter
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.03.2011
• Subjects: 2-Naphthylamine - analogs & derivatives; Amides - chemistry; Bilayer membrane; Calorimetry, Differential Scanning; Cell Membrane - chemistry; Cholesterol; Cholesterol - chemistry; Fatty Acids - chemistry; Fluorescent Dyes; Hydroxylated sphingomyelin; Hydroxylation; Lateral interaction; Laurates; Lipid Bilayers - chemistry; Ordered domains; Phospholipids - chemistry; Sphingomyelins - chemistry; Sterol affinity; Sterols - chemistry; Temperature; 22:0-SM; Hydroxylated sphingomyelin; Sterol affinity; PSM; Ordered domains; Lateral interaction; 7SLPC; Cholesterol; 3OH-PSM; 2OH-22:0-SM; POPC; 2OH-PSM; CTL; DPH; Bilayer membrane

The bilayer properties and interactions with cholesterol of N-acyl hydroxylated sphingomyelins (SM) were examined, and results were compared to nonhydroxylated chain-matched SM. The natural OH(d)-enantiomer of hydroxylated SM (with 16:0 or 22:0 acyl chain lengths) analogs was synthesized. Measuring...