A global investigation of gene deletion strains that affect premature stop codon bypass in yeast, Saccharomyces cerevisiae

• Published in: Molecular bioSystems Vol. 10; no. 4; pp. 916 - 924
• Main Authors: Samanfar, Bahram, Tan, Le Hoa, Shostak, Kristina, Chalabian, Firoozeh, Wu, Zongbin, Alamgir, Md, Sunba, Noor, Burnside, Daniel, Omidi, Katayoun, Hooshyar, Mohsen, Galván Márquez, Imelda, Jessulat, Matthew, Smith, Myron L, Babu, Mohan, Azizi, Ali, Golshani, Ashkan
• Format: Journal Article
• Language: English
• Published: England 01.01.2014
• Subjects: Adenosine Triphosphatases - biosynthesis; Adenosine Triphosphatases - genetics; beta-Galactosidase - biosynthesis; beta-Galactosidase - genetics; Codon, Nonsense - genetics; Gene Deletion; Lac Operon - genetics; Plasmids - genetics; Protein Biosynthesis - genetics; Saccharomyces cerevisiae; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae Proteins - biosynthesis; Saccharomyces cerevisiae Proteins - genetics; Solute Carrier Family 12, Member 2 - biosynthesis; Solute Carrier Family 12, Member 2 - genetics

Protein biosynthesis is an orderly process that requires a balance between rate and accuracy. To produce a functional product, the fidelity of this process has to be maintained from start to finish. In order to systematically identify genes that affect stop codon bypass, three expression plasmids, p...