Aggrecan synthesized by mature bovine chondrocytes suspended in alginate. Identification of two distinct metabolic matrix pools

• Published in: The Journal of biological chemistry Vol. 269; no. 52; pp. 33021 - 33027
• Main Authors: S S Mok, K Masuda, H J Häuselmann, M B Aydelotte, E J Thonar
• Format: Journal Article
• Language: English
• Published: United States American Society for Biochemistry and Molecular Biology 30.12.1994
• Subjects: Aggrecans; Alginates; Animals; Autoradiography; Cartilage, Articular - cytology; Cartilage, Articular - metabolism; Cattle; Cells, Cultured; Chondroitin Sulfate Proteoglycans - biosynthesis; Chondroitin Sulfate Proteoglycans - metabolism; Extracellular Matrix Proteins; Glucuronic Acid; Hexuronic Acids; Kinetics; Lectins, C-Type; Proteoglycans - biosynthesis; Proteoglycans - metabolism

Proteoglycans synthesized by chondrocytes in alginate beads are found in two compartments: the cell-associated matrix and the further removed matrix (Häuselmann, H. J., Aydelotte M. B., Schumacher B. L., Kuettner K. E., Gitelis, S. H., and Thonar, E. J.-M. A. (1992) Matrix 12, 116-129). To study th...