New multiplex conventional PCR and quadruplex real-time PCR assays for one-tube detection of Phyllosticta citricarpa, Elsinoë fawcettii, Elsinoë australis, and Pseudocercospora angolensis in Citrus: development and validation

• Published in: Applied microbiology and biotechnology Vol. 104; no. 21; pp. 9363 - 9385
• Main Authors: Ahmed, Yosra, Hussein, Ahmed, Hubert, Jacqueline, Fourrier-Jeandel, Céline, Aguayo, Jaime, Ioos, Renaud
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.11.2020; Springer Nature B.V
• Subjects: Ascomycota; Assaying; Biomedical and Life Sciences; Biotechnology; Citrus; cost effectiveness; Cross-reaction; Deoxyribonucleic acid; detection limit; DNA; DNA probes; Extraction procedures; Fruits; fungi; Guignardia citricarpa; hydrolysis; Life Sciences; Methods and Protocols; Microbial Genetics and Genomics; Microbiology; Multiplex Polymerase Chain Reaction; Multiplexing; Oligonucleotides; Pathogens; Phyllosticta citricarpa; Plant Diseases; Plant tissues; Polymerase chain reaction; Pseudocercospora; quantitative polymerase chain reaction; Real time; Real-Time Polymerase Chain Reaction; Regulatory agencies; Reproducibility; Reproducibility of Results; Robustness (mathematics); Sensitivity; Pseudocercospora leaf and fruit spot; Citrus black spot; Citrus scab; Citrus quarantine pathogens

Phyllosticta citricarpa , Elsinoë fawcettii , Elsinoë australis , and Pseudocercospora angolensis are major pathogens of citrus crops worldwide and can cause non-characteristic symptoms that may lead to confusion regarding the causative agent . These fungi are subject to international phytosanitary...