Determination of meropenem in serum by high-performance liquid chromatography with column switching

A rapid, accurate and sensitive liquid chromatographic assay with on-line solid-phase extraction for determination of meropenem in serum is described. Sample was directly injected onto the extraction column for sample clean-up and extraction. Thereafter, using an on-line column-switching system the...

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Bibliographic Details
Published inJournal of Chromatography A Vol. 812; no. 1; pp. 249 - 253
Main Authors Bompadre, S, Ferrante, L, De Martinis, M, Leone, L
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 03.07.1998
Subjects
Antibiotics
Chromatography, High Pressure Liquid
Humans
Indicators and Reagents
Meropenem
Solvents
Spectrophotometry, Ultraviolet
Thienamycins - blood
Thienamycins - pharmacokinetics
Meropenem
Column switching
Sample preparation
Antibiotics
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ISSN0021-9673
DOI10.1016/S0021-9673(98)00249-0

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Summary:A rapid, accurate and sensitive liquid chromatographic assay with on-line solid-phase extraction for determination of meropenem in serum is described. Sample was directly injected onto the extraction column for sample clean-up and extraction. Thereafter, using an on-line column-switching system the drug was quantitatively transferred and separated on a C 18 analytical column. Ultraviolet absorption at 298 nm was used for detection. The assay was linear from 1 to 100 μg/ml. Recovery was 98.5%. Based on a 20-μl sample volume (serum–water, 1:1, v/v), detection limit was 0.1 μg/ml. An application of the method to study the pharmacokinetics of meropenem is given.
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ISSN:0021-9673
DOI:10.1016/S0021-9673(98)00249-0