Use of cultured cells as a control for quantitative immunocytochemical analysis of estrogen receptor in breast cancer: The Quicgel method

• Published in: American journal of clinical pathology Vol. 111; no. 3; pp. 329 - 335
• Main Authors: RIERA, J, SIMPSON, J. F, TAMAYO, R, BATTIFORA, H, MD
• Format: Journal Article
• Language: English
• Published: Chicago, IL American Society of Clinical Pathologists 01.03.1999
• Subjects: Adult; Aged; Aged, 80 and over; Biological and medical sciences; Breast Neoplasms - chemistry; Breast Neoplasms - pathology; Carcinoma - chemistry; Carcinoma - pathology; Cell Count; Female; Humans; Image Processing, Computer-Assisted; Immunoenzyme Techniques; Investigative techniques, diagnostic techniques (general aspects); Medical sciences; Middle Aged; Miscellaneous. Technology; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Quality Control; Receptors, Estrogen - analysis; Reproducibility of Results; Tumor Cells, Cultured - chemistry; Tumor Cells, Cultured - pathology; Human; Immunohistochemistry; Cell culture; Carcinoma; Estrogen; Malignant tumor; Ovarian hormone; Pathology; Mammary gland diseases; Quality control; Established cell line; Mammary gland; Technique; Sex steroid hormone; Freeze; Quantitative analysis; Hormonal receptor

Variation in tissue fixation, processing, and staining is largely responsible for poor reproducibility of estrogen receptor (ER) immunohistochemistry assays. A frozen, agar-suspended pellet of MCF-7 cells with known ER content was added to each of 55 samples of invasive breast carcinoma (IBC), servi...