Strong CD4+ T-Cell Responses to Ancestral and Variant Spike Proteins Are Established by NVX-CoV2373 Severe Acute Respiratory Syndrome Coronavirus 2 Primary Vaccination

Abstract Background NVX-CoV2373 is an efficacious coronavirus disease 2019 (COVID-19) vaccine comprising full-length recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (rS) glycoprotein and Matrix-M adjuvant. Phase 2 of a randomized, placebo-controlled, phase 1/2 trial in...

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Published inThe Journal of infectious diseases Vol. 228; no. 6; pp. 734 - 741
Main Authors Fries, Louis, Formica, Neil, Mallory, Raburn M, Zhou, Haixia, Plested, Joyce S, Kalkeri, Raj, Moldovan, Ioana, Patel, Nita, Albert, Gary, Robinson, Michelle, Cho, Iksung, Chau, Gordon, Dubovsky, Filip, Glenn, Gregory M
Format Journal Article
LanguageEnglish
Published Oxford Oxford University Press 15.09.2023
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Abstract Abstract Background NVX-CoV2373 is an efficacious coronavirus disease 2019 (COVID-19) vaccine comprising full-length recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (rS) glycoprotein and Matrix-M adjuvant. Phase 2 of a randomized, placebo-controlled, phase 1/2 trial in healthy adults (18–84 years of age) previously reported good safety/tolerability and robust humoral immunogenicity. Methods Participants were randomized to placebo or 1 or 2 doses of 5-µg or 25-µg rS with 50 µg Matrix-M adjuvant 21 days apart. CD4+ T-cell responses to SARS-CoV-2 intact S or pooled peptide stimulation (with ancestral or variant S sequences) were measured via enzyme-linked immunosorbent spot assay and intracellular cytokine staining. Results A clearly discernable spike antigen-specific CD4+ T-cell response was induced after 1 dose, but markedly enhanced after 2 doses. Counts and fold increases in cells producing Th1 cytokines exceeded those secreting Th2 cytokines, although both phenotypes were clearly present. Interferon-γ responses to rS were detected in 93.5% of 2-dose 5-µg recipients. A polyfunctional CD4+ T-cell response was cross-reactive and of equivalent magnitude to all tested variants, including Omicron BA.1/BA.5. Conclusions NVX-CoV2373 elicits a moderately Th1-biased CD4+ T-cell response that is cross-reactive with ancestral and variant S proteins after 2 doses. Clinical Trials Registration NCT04368988. Primary series of NVX-CoV2373 vaccine with Matrix-M adjuvant induces polyfunctional CD4+ T-cell responses of balanced to moderately Th1-biased phenotype. Essentially equivalent cytokine production by vaccinee cells can be stimulated by spike proteins of ancestral or multiple variant sequences.
AbstractList Primary series of NVX-CoV2373 vaccine with Matrix-M adjuvant induces polyfunctional CD4 + T-cell responses of balanced to moderately Th1-biased phenotype. Essentially equivalent cytokine production by vaccinee cells can be stimulated by spike proteins of ancestral or multiple variant sequences.
Background NVX-CoV2373 is an efficacious coronavirus disease 2019 (COVID-19) vaccine comprising full-length recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (rS) glycoprotein and Matrix-M adjuvant. Phase 2 of a randomized, placebo-controlled, phase 1/2 trial in healthy adults (18–84 years of age) previously reported good safety/tolerability and robust humoral immunogenicity. Methods Participants were randomized to placebo or 1 or 2 doses of 5-µg or 25-µg rS with 50 µg Matrix-M adjuvant 21 days apart. CD4+ T-cell responses to SARS-CoV-2 intact S or pooled peptide stimulation (with ancestral or variant S sequences) were measured via enzyme-linked immunosorbent spot assay and intracellular cytokine staining. Results A clearly discernable spike antigen-specific CD4+ T-cell response was induced after 1 dose, but markedly enhanced after 2 doses. Counts and fold increases in cells producing Th1 cytokines exceeded those secreting Th2 cytokines, although both phenotypes were clearly present. Interferon-γ responses to rS were detected in 93.5% of 2-dose 5-µg recipients. A polyfunctional CD4+ T-cell response was cross-reactive and of equivalent magnitude to all tested variants, including Omicron BA.1/BA.5. Conclusions NVX-CoV2373 elicits a moderately Th1-biased CD4+ T-cell response that is cross-reactive with ancestral and variant S proteins after 2 doses. Clinical Trials Registration NCT04368988.
Abstract Background NVX-CoV2373 is an efficacious coronavirus disease 2019 (COVID-19) vaccine comprising full-length recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (rS) glycoprotein and Matrix-M adjuvant. Phase 2 of a randomized, placebo-controlled, phase 1/2 trial in healthy adults (18–84 years of age) previously reported good safety/tolerability and robust humoral immunogenicity. Methods Participants were randomized to placebo or 1 or 2 doses of 5-µg or 25-µg rS with 50 µg Matrix-M adjuvant 21 days apart. CD4+ T-cell responses to SARS-CoV-2 intact S or pooled peptide stimulation (with ancestral or variant S sequences) were measured via enzyme-linked immunosorbent spot assay and intracellular cytokine staining. Results A clearly discernable spike antigen-specific CD4+ T-cell response was induced after 1 dose, but markedly enhanced after 2 doses. Counts and fold increases in cells producing Th1 cytokines exceeded those secreting Th2 cytokines, although both phenotypes were clearly present. Interferon-γ responses to rS were detected in 93.5% of 2-dose 5-µg recipients. A polyfunctional CD4+ T-cell response was cross-reactive and of equivalent magnitude to all tested variants, including Omicron BA.1/BA.5. Conclusions NVX-CoV2373 elicits a moderately Th1-biased CD4+ T-cell response that is cross-reactive with ancestral and variant S proteins after 2 doses. Clinical Trials Registration NCT04368988. Primary series of NVX-CoV2373 vaccine with Matrix-M adjuvant induces polyfunctional CD4+ T-cell responses of balanced to moderately Th1-biased phenotype. Essentially equivalent cytokine production by vaccinee cells can be stimulated by spike proteins of ancestral or multiple variant sequences.
BACKGROUNDNVX-CoV2373 is an efficacious coronavirus disease 2019 (COVID-19) vaccine comprising full-length recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (rS) glycoprotein and Matrix-M adjuvant. Phase 2 of a randomized, placebo-controlled, phase 1/2 trial in healthy adults (18-84 years of age) previously reported good safety/tolerability and robust humoral immunogenicity. METHODSParticipants were randomized to placebo or 1 or 2 doses of 5-µg or 25-µg rS with 50 µg Matrix-M adjuvant 21 days apart. CD4+ T-cell responses to SARS-CoV-2 intact S or pooled peptide stimulation (with ancestral or variant S sequences) were measured via enzyme-linked immunosorbent spot assay and intracellular cytokine staining. RESULTSA clearly discernable spike antigen-specific CD4+ T-cell response was induced after 1 dose, but markedly enhanced after 2 doses. Counts and fold increases in cells producing Th1 cytokines exceeded those secreting Th2 cytokines, although both phenotypes were clearly present. Interferon-γ responses to rS were detected in 93.5% of 2-dose 5-µg recipients. A polyfunctional CD4+ T-cell response was cross-reactive and of equivalent magnitude to all tested variants, including Omicron BA.1/BA.5. CONCLUSIONSNVX-CoV2373 elicits a moderately Th1-biased CD4+ T-cell response that is cross-reactive with ancestral and variant S proteins after 2 doses. CLINICAL TRIALS REGISTRATIONNCT04368988.
Author Moldovan, Ioana
Albert, Gary
Patel, Nita
Formica, Neil
Robinson, Michelle
Mallory, Raburn M
Dubovsky, Filip
Glenn, Gregory M
Plested, Joyce S
Kalkeri, Raj
Chau, Gordon
Zhou, Haixia
Cho, Iksung
Fries, Louis
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Cites_doi 10.1126/sciimmunol.abo2202
10.1056/NEJMc2215509
10.1093/cid/ciac803
10.1016/S1473-3099(22)00309-7
10.1016/j.vaccine.2016.02.033
10.1016/j.cell.2021.12.033
10.1016/j.vaccine.2017.08.021
10.3390/cells6030029
10.1093/cid/ciaa1673
10.1016/j.xcrm.2021.100354
10.1016/j.vaccine.2015.06.047
10.3389/fimmu.2022.882972
10.1056/NEJMc1313186
10.1056/NEJMoa2026920
10.1056/NEJMoa2116185
10.1038/nm.2612
10.1093/infdis/jiz518
10.1016/j.vaccine.2021.09.052
10.1371/journal.pmed.1003769
10.1016/S0140-6736(20)32661-1
10.1056/NEJMoa2107659
10.1056/NEJMoa2107715
10.1172/JCI160898
10.1038/s41467-020-20653-8
10.1056/NEJMoa2034577
10.1016/S1473-3099(22)00420-0
10.1186/s40249-021-00915-3
10.1016/j.cell.2022.05.022
10.1038/s41467-022-35606-6
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Issue 6
Keywords COVID-19 vaccine
CD4
variant cross-reactivity
polyfunctional
Matrix-M adjuvant
T-cell response
Language English
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Potential conflicts of interest. L. F., R. R. M., H. Z., J. S. P., R. K., N. P., G. A., M. R., I. C., G. C., F. D., and G. M. G. are all salaried employees or contractors of Novavax. N. F. was a contractor for Novavax at the time this study was done, and is currently employed at Formative Health Pty Ltd, Casuarina NSW 2487, Australia. I. M. is an employee of Cellular Technology Ltd.
Present affiliation: Formative Health Pty Ltd, Casuarina, New South Wales, Australia.
Membership of the 2019nCoV-101 Study Group is provided in the Acknowledgments and in Supplementary Table 1.
All authors have submitted the ICMJE Form for Disclosure of Potential Conflicts of Interest. Conflicts that the editors consider relevant to the content of the manuscript have been disclosed.
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References Rydyznski Moderbacher (2023091519573497200_jiad163-B16) 2022; 132
Logue (2023091519573497200_jiad163-B28) 2023; 14
Chung (2023091519573497200_jiad163-B13) 2015; 33
Fries (2023091519573497200_jiad163-B11) 2020; 222
Alves (2023091519573497200_jiad163-B29) 2023; 388
Voysey (2023091519573497200_jiad163-B3) 2021; 397
Polack (2023091519573497200_jiad163-B2) 2020; 383
GeurtsvanKessel (2023091519573497200_jiad163-B8) 2022; 7
Gartlan (2023091519573497200_jiad163-B24) 2022; 13
Dunkle (2023091519573497200_jiad163-B22) 2022; 386
Fadlyana (2023091519573497200_jiad163-B1) 2021; 39
Liu (2023091519573497200_jiad163-B5) 2021; 10
Tian (2023091519573497200_jiad163-B17) 2021; 12
Garcia-Beltran (2023091519573497200_jiad163-B7) 2022; 185
Formica (2023091519573497200_jiad163-B19) 2021; 18
Heath (2023091519573497200_jiad163-B20) 2021; 385
Smith (2023091519573497200_jiad163-B15) 2017; 35
Keech (2023091519573497200_jiad163-B10) 2020; 383
Duechting (2023091519573497200_jiad163-B27) 2017; 6
Bengtsson (2023091519573497200_jiad163-B9) 2016; 34
Jara (2023091519573497200_jiad163-B4) 2021; 385
Shinde (2023091519573497200_jiad163-B14) 2021; 73
Mallory (2023091519573497200_jiad163-B23) 2022; 22
Kirsebom (2023091519573497200_jiad163-B6) 2022; 22
Heath (2023091519573497200_jiad163-B21) 2023; 76
Zhang (2023091519573497200_jiad163-B18) 2022; 185
Wilkinson (2023091519573497200_jiad163-B26) 2012; 18
Cohen (2023091519573497200_jiad163-B25) 2021; 2
Fries (2023091519573497200_jiad163-B12) 2013; 369
References_xml – volume: 7
  year: 2022
  ident: 2023091519573497200_jiad163-B8
  article-title: Divergent SARS-CoV-2 Omicron-reactive T and B cell responses in COVID-19 vaccine recipients
  publication-title: Sci Immunol
  doi: 10.1126/sciimmunol.abo2202
  contributor:
    fullname: GeurtsvanKessel
– volume: 388
  start-page: 857
  year: 2023
  ident: 2023091519573497200_jiad163-B29
  article-title: Immunogenicity of a fourth homologous dose of NVX-CoV2373
  publication-title: N Engl J Med
  doi: 10.1056/NEJMc2215509
  contributor:
    fullname: Alves
– volume: 76
  start-page: 398
  year: 2023
  ident: 2023091519573497200_jiad163-B21
  article-title: Safety and efficacy of the NVX-CoV2373 coronavirus disease 2019 vaccine at completion of the placebo-controlled phase of a randomized controlled trial
  publication-title: Clin Infect Dis
  doi: 10.1093/cid/ciac803
  contributor:
    fullname: Heath
– volume: 22
  start-page: 931
  year: 2022
  ident: 2023091519573497200_jiad163-B6
  article-title: COVID-19 vaccine effectiveness against the Omicron (BA.2) variant in England
  publication-title: Lancet Infect Dis
  doi: 10.1016/S1473-3099(22)00309-7
  contributor:
    fullname: Kirsebom
– volume: 34
  start-page: 1927
  year: 2016
  ident: 2023091519573497200_jiad163-B9
  article-title: Matrix-M adjuvant enhances antibody, cellular and protective immune responses of a Zaire Ebola/Makona virus glycoprotein (GP) nanoparticle vaccine in mice
  publication-title: Vaccine
  doi: 10.1016/j.vaccine.2016.02.033
  contributor:
    fullname: Bengtsson
– volume: 185
  start-page: 457
  year: 2022
  ident: 2023091519573497200_jiad163-B7
  article-title: mRNA-based COVID-19 vaccine boosters induce neutralizing immunity against SARS-CoV-2 Omicron variant
  publication-title: Cell
  doi: 10.1016/j.cell.2021.12.033
  contributor:
    fullname: Garcia-Beltran
– volume: 35
  start-page: 5366
  year: 2017
  ident: 2023091519573497200_jiad163-B15
  article-title: Novel hemagglutinin nanoparticle influenza vaccine with Matrix-M adjuvant induces hemagglutination inhibition, neutralizing, and protective responses in ferrets against homologous and drifted A(H3N2) subtypes
  publication-title: Vaccine
  doi: 10.1016/j.vaccine.2017.08.021
  contributor:
    fullname: Smith
– volume: 6
  start-page: 29
  year: 2017
  ident: 2023091519573497200_jiad163-B27
  article-title: Delayed activation kinetics of Th2- and Th17 cells compared to Th1 cells
  publication-title: Cells
  doi: 10.3390/cells6030029
  contributor:
    fullname: Duechting
– volume: 73
  start-page: e4278
  year: 2021
  ident: 2023091519573497200_jiad163-B14
  article-title: Induction of cross-reactive hemagglutination inhibiting antibody and polyfunctional CD4+ T-cell responses by a recombinant Matrix-M–adjuvanted hemagglutinin nanoparticle influenza vaccine
  publication-title: Clin Infec Dis
  doi: 10.1093/cid/ciaa1673
  contributor:
    fullname: Shinde
– volume: 2
  start-page: 100354
  year: 2021
  ident: 2023091519573497200_jiad163-B25
  article-title: Longitudinal analysis shows durable and broad immune memory after SARS-CoV-2 infection with persisting antibody responses and memory B and T cells
  publication-title: Cell Rep Med
  doi: 10.1016/j.xcrm.2021.100354
  contributor:
    fullname: Cohen
– volume: 33
  start-page: 3953
  year: 2015
  ident: 2023091519573497200_jiad163-B13
  article-title: ISCOMATRIX adjuvant promotes epitope spreading and antibody affinity maturation of influenza A H7N9 virus like particle vaccine that correlate with virus neutralization in humans
  publication-title: Vaccine
  doi: 10.1016/j.vaccine.2015.06.047
  contributor:
    fullname: Chung
– volume: 13
  start-page: 882972
  year: 2022
  ident: 2023091519573497200_jiad163-B24
  article-title: Vaccine-associated enhanced disease and pathogenic human coronaviruses
  publication-title: Front Immunol
  doi: 10.3389/fimmu.2022.882972
  contributor:
    fullname: Gartlan
– volume: 369
  start-page: 2564
  year: 2013
  ident: 2023091519573497200_jiad163-B12
  article-title: A recombinant viruslike particle influenza A (H7N9) vaccine
  publication-title: N Engl J Med
  doi: 10.1056/NEJMc1313186
  contributor:
    fullname: Fries
– volume: 383
  start-page: 2320
  year: 2020
  ident: 2023091519573497200_jiad163-B10
  article-title: Phase 1–2 trial of a SARS-CoV-2 recombinant spike protein nanoparticle vaccine
  publication-title: N Engl J Med
  doi: 10.1056/NEJMoa2026920
  contributor:
    fullname: Keech
– volume: 386
  start-page: 531
  year: 2022
  ident: 2023091519573497200_jiad163-B22
  article-title: Efficacy and safety of NVX-CoV2373 in adults in the United States and Mexico
  publication-title: N Engl J Med
  doi: 10.1056/NEJMoa2116185
  contributor:
    fullname: Dunkle
– volume: 18
  start-page: 274
  year: 2012
  ident: 2023091519573497200_jiad163-B26
  article-title: Preexisting influenza-specific CD4+ T cells correlate with disease protection against influenza challenge in humans
  publication-title: Nat Med
  doi: 10.1038/nm.2612
  contributor:
    fullname: Wilkinson
– volume: 222
  start-page: 572
  year: 2020
  ident: 2023091519573497200_jiad163-B11
  article-title: Randomized, blinded, dose-ranging trial of an Ebola virus glycoprotein nanoparticle vaccine with Matrix-M adjuvant in healthy adults
  publication-title: J Infect Dis
  doi: 10.1093/infdis/jiz518
  contributor:
    fullname: Fries
– volume: 39
  start-page: 6520
  year: 2021
  ident: 2023091519573497200_jiad163-B1
  article-title: A phase III, observer-blind, randomized, placebo-controlled study of the efficacy, safety, and immunogenicity of SARS-CoV-2 inactivated vaccine in healthy adults aged 18–59 years: an interim analysis in Indonesia
  publication-title: Vaccine
  doi: 10.1016/j.vaccine.2021.09.052
  contributor:
    fullname: Fadlyana
– volume: 18
  year: 2021
  ident: 2023091519573497200_jiad163-B19
  article-title: Different dose regimens of a SARS-CoV-2 recombinant spike protein vaccine (NVX-CoV2373) in younger and older adults: a phase 2 randomized placebo-controlled trial
  publication-title: PLoS Medicine
  doi: 10.1371/journal.pmed.1003769
  contributor:
    fullname: Formica
– volume: 397
  start-page: 99
  year: 2021
  ident: 2023091519573497200_jiad163-B3
  article-title: Safety and efficacy of the ChAdOx1 nCoV-19 vaccine (AZD1222) against SARS-CoV-2: an interim analysis of four randomised controlled trials in Brazil, South Africa, and the UK
  publication-title: Lancet
  doi: 10.1016/S0140-6736(20)32661-1
  contributor:
    fullname: Voysey
– volume: 385
  start-page: 1172
  year: 2021
  ident: 2023091519573497200_jiad163-B20
  article-title: Safety and efficacy of NVX-CoV2373 Covid-19 vaccine
  publication-title: N Engl J Med
  doi: 10.1056/NEJMoa2107659
  contributor:
    fullname: Heath
– volume: 385
  start-page: 875
  year: 2021
  ident: 2023091519573497200_jiad163-B4
  article-title: Effectiveness of an inactivated SARS-CoV-2 vaccine in Chile
  publication-title: N Engl J Med
  doi: 10.1056/NEJMoa2107715
  contributor:
    fullname: Jara
– volume: 132
  year: 2022
  ident: 2023091519573497200_jiad163-B16
  article-title: NVX-CoV2373 vaccination induces functional SARS-CoV-2–specific CD4+ and CD8+ T cell responses
  publication-title: J Clin Invest
  doi: 10.1172/JCI160898
  contributor:
    fullname: Rydyznski Moderbacher
– volume: 12
  start-page: 372
  year: 2021
  ident: 2023091519573497200_jiad163-B17
  article-title: SARS-CoV-2 spike glycoprotein vaccine candidate NVX-CoV2373 immunogenicity in baboons and protection in mice
  publication-title: Nat Comm
  doi: 10.1038/s41467-020-20653-8
  contributor:
    fullname: Tian
– volume: 383
  start-page: 2603
  year: 2020
  ident: 2023091519573497200_jiad163-B2
  article-title: Safety and efficacy of the BNT162b2 mRNA Covid-19 vaccine
  publication-title: N Engl J Med
  doi: 10.1056/NEJMoa2034577
  contributor:
    fullname: Polack
– volume: 22
  start-page: 1565
  year: 2022
  ident: 2023091519573497200_jiad163-B23
  article-title: Safety and immunogenicity following a homologous booster dose of a SARS-CoV-2 recombinant spike protein vaccine (NVX-CoV2373): a secondary analysis of a randomised, placebo-controlled, phase 2 trial
  publication-title: Lancet Infect Dis
  doi: 10.1016/S1473-3099(22)00420-0
  contributor:
    fullname: Mallory
– volume: 10
  start-page: 132
  year: 2021
  ident: 2023091519573497200_jiad163-B5
  article-title: Effectiveness and safety of SARS-CoV-2 vaccine in real-world studies: a systematic review and meta-analysis
  publication-title: Infect Dis Poverty
  doi: 10.1186/s40249-021-00915-3
  contributor:
    fullname: Liu
– volume: 185
  start-page: 2434
  year: 2022
  ident: 2023091519573497200_jiad163-B18
  article-title: Humoral and cellular immune memory to four COVID-19 vaccines
  publication-title: Cell
  doi: 10.1016/j.cell.2022.05.022
  contributor:
    fullname: Zhang
– volume: 14
  start-page: 1130
  year: 2023
  ident: 2023091519573497200_jiad163-B28
  article-title: Immunogenicity and protection of a variant nanoparticle vaccine that confers broad neutralization against SARS-CoV-2 variants
  publication-title: Nat Commun
  doi: 10.1038/s41467-022-35606-6
  contributor:
    fullname: Logue
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Snippet Abstract Background NVX-CoV2373 is an efficacious coronavirus disease 2019 (COVID-19) vaccine comprising full-length recombinant severe acute respiratory...
Background NVX-CoV2373 is an efficacious coronavirus disease 2019 (COVID-19) vaccine comprising full-length recombinant severe acute respiratory syndrome...
BACKGROUNDNVX-CoV2373 is an efficacious coronavirus disease 2019 (COVID-19) vaccine comprising full-length recombinant severe acute respiratory syndrome...
Primary series of NVX-CoV2373 vaccine with Matrix-M adjuvant induces polyfunctional CD4 + T-cell responses of balanced to moderately Th1-biased phenotype....
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SubjectTerms CD4 antigen
Clinical trials
Coronaviruses
COVID-19
Cytokines
Immunogenicity
Lymphocytes T
Major
Phenotypes
Placebos
Severe acute respiratory syndrome coronavirus 2
γ-Interferon
Title Strong CD4+ T-Cell Responses to Ancestral and Variant Spike Proteins Are Established by NVX-CoV2373 Severe Acute Respiratory Syndrome Coronavirus 2 Primary Vaccination
URI https://www.proquest.com/docview/2955299607
https://search.proquest.com/docview/2817779230
https://pubmed.ncbi.nlm.nih.gov/PMC10503953
Volume 228
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