Proteolytic processing of a secreted glycoprotein and O-glycosylation of mannoproteins are affected in the N-glycosylation mutant Saccharomyces cerevisiae ldb1

• Published in: Biochimica et biophysica acta Vol. 1380; no. 3; pp. 320 - 328
• Main Authors: Mañas, Paula, Olivero, Isabel, Hernández, Luis M
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 08.05.1998
• Subjects: beta-Glucosidase - genetics; beta-Glucosidase - metabolism; Carbohydrate Conformation; Glucan 1,3-beta-Glucosidase; Glycoproteins - genetics; Glycoproteins - metabolism; Glycosylation; Golgi Apparatus - genetics; Golgi Apparatus - metabolism; Isoenzymes - chemistry; Isoenzymes - genetics; Isoenzymes - metabolism; ldb1 mutant; Mannoprotein; Membrane Glycoproteins - genetics; Membrane Glycoproteins - metabolism; Mutation; N-glycosylation; O-glycosylation; Oligosaccharides - analysis; Oligosaccharides - genetics; Protein Processing, Post-Translational - genetics; Proteolytic processing; Saccharomyces cerevisiae; Saccharomyces cerevisiae - enzymology; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; DPAP A, dipeptidyl aminopeptidase A; TLC, thin layer chromatography; TGN, trans-Golgi network; O-glycosylation; p-NPG, p-nitrophenyl- β- d-glucopyranoside; Mannoprotein; N-glycosylation; Proteolytic processing; endo H, endo- β- N-acetylglucosaminidase H; ldb1 mutant; PAGE, polyacrylamide gel electrophoresis; Saccharomyces cerevisiae; ER, endoplasmic reticulum

In a previous work [P.I. Mañas, I. Olivero, M. Avalos, L.M. Hernández, Glycobiology, 7 (1997) 487–497], we described the isolation and characterization of the Saccharomyces cerevisiae ldb1 mutant which is affected in several steps of the N-glycosylation of mannoproteins probably due to a malfunction...