Exonuclease enhances hybridization efficiency: Improved direct cycle sequencing and point mutation detection

• Published in: Biochimica et biophysica acta Vol. 1379; no. 1; pp. 42 - 52
• Main Authors: Somers, Veerle A.M.C, Leimbach, Darcy A, Murtagh, James J, Thunnissen, Frederik B.J.M
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 08.01.1998
• Subjects: DNA Mutational Analysis; DNA Probes - chemistry; Exodeoxyribonucleases - metabolism; Exonuclease; Fluorescence; Genes, ras - genetics; Genetic Testing; Heterozygosity; Heterozygote; Humans; Neoplasms - genetics; Nucleic Acid Hybridization - methods; Point mutation; Point Mutation - genetics; Ras; Sensitivity; Sequence Analysis, DNA - methods; Sequencing; Exonuclease; Sensitivity; Ras; Sequencing; Point mutation; Heterozygosity

Solution hybridization is an essential step in sequencing and some point mutation detection methods. In practice, this hybridization is hampered resulting in the need of additional purification of the amplification products. The use of T7 gene 6 exonuclease may lead to efficient production of single...