UVB-Induced Senescence of Human Dermal Fibroblasts Involves Impairment of Proteasome and Enhanced Autophagic Activity

• Published in: The journals of gerontology. Series A, Biological sciences and medical sciences Vol. 72; no. 5; p. 632
• Main Authors: Cavinato, Maria, Koziel, Rafal, Romani, Nikolaus, Weinmüllner, Regina, Jenewein, Brigitte, Hermann, Martin, Dubrac, Sandrine, Ratzinger, Gudrun, Grillari, Johannes, Schmuth, Matthias, Jansen-Dürr, Pidder
• Format: Journal Article
• Language: English
• Published: United States 01.05.2017
• Subjects: Autophagy - radiation effects; Blotting, Western; Cell Proliferation - radiation effects; Cells, Cultured; Cellular Senescence - radiation effects; Dose-Response Relationship, Radiation; Fibroblasts - radiation effects; Humans; Proteasome Endopeptidase Complex - radiation effects; Radioimmunoprecipitation Assay; Reactive Oxygen Species - metabolism; Real-Time Polymerase Chain Reaction; Skin Aging - radiation effects; Ultraviolet Rays; UVB; Senescence; Skin aging; Autophagy; Proteasome

In the current study, we have extended previous findings aiming at a better understanding of molecular mechanisms underlying UVB-induced senescence of diploid human dermal fibroblasts (HDFs), an experimental model to study the process of photoaging in the skin. We provide evidence that the inhibitio...