Development and validation of an enzyme linked immunosorbent assay for fluoroquinolones in animal feeds

• Published in: Food control Vol. 57; pp. 195 - 201
• Main Authors: Tufa, Ramato Ashu, Pinacho, Daniel G., Pascual, Núria, Granados, Mercè, Companyó, Ramon, Marco, M. Pilar
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.11.2015
• Subjects: ELISA; Feed analysis; Method validation; Quinolones; Screening method; Screening method; Method validation; Feed analysis; ELISA; Quinolones

An enzyme-linked immunosorbent assay (ELISA) for analysis of fluoroquinolones residues in animal feeds has been developed and validated according to Commission Decision 2002/657/EC criteria. Initially, direct and indirect competitive ELISA formats were compared for one fluoroquinolone polyclonal ant...