Optical and electron paramagnetic resonance absorption spectra of complexes of nitric oxide synthase I with isocyanides

• Published in: Biochimica et biophysica acta Vol. 1335; no. 3; pp. 253 - 264
• Main Authors: Yoneyama, Hirohito, Hori, Hiroshi, Ichikawa, Yoshiyuki
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 06.06.1997
• Subjects: Animals; Binding Sites; Brain - enzymology; Cyanides - chemistry; Cytochrome P-450; Cytochrome P-450 Enzyme System - chemistry; Electron Spin Resonance Spectroscopy; Heme - chemistry; Hydrogen-Ion Concentration; Microsomes, Liver - enzymology; Monooxygenase; Nitric oxide synthase; Nitric Oxide Synthase - chemistry; Nitric Oxide Synthase - isolation & purification; Nitric oxide synthase I-isocyanide complex; Oxidation-Reduction; Phenylisocyanide; Rabbits; Spectrophotometry; Swine; EDTA, ethylenediamine tetraacetate dihydrate; Phenylisocyanide; NOS I, neuronal nitric oxide synthase; Nitric oxide synthase I-isocyanide complex; EPR, electron paramagnetic resonance; MOPS, 3-[ N-morpholino]propanesulfonic acid; Cytochrome P-450; Nitric oxide synthase; NOS III, endothelial nitric oxide synthase; NOS, nitric oxide synthase; Monooxygenase; NAME, N ω -nitro- l-arginine methyl ester

Neuronal nitric oxide synthase (NOS I) was purified from porcine brains, and optical and EPR spectra of the complexes of NOS I with isocyanides were investigated. The complex of oxidized NOS I with tert-butylisocyanide exhibited optical absorption peaks at 437 and 560 nm in the difference spectrum,...