Intracellular stimulation and recording from single cardiac cells in the rabbit atrium
The mechanism of pacemaker action in an isolated rabbit sinoatrial node preparation was studied by means of intracellular stimulation and recording through a single-barrelled microelectrode. This technique permitted not only stimulation of individual cells of the various types found in and around th...
Saved in:
Published in | Journal of electrocardiology Vol. 7; no. 2; pp. 119 - 126 |
---|---|
Main Authors | , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
1974
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | The mechanism of pacemaker action in an isolated rabbit sinoatrial node preparation was studied by means of intracellular stimulation and recording through a single-barrelled microelectrode.
This technique permitted not only stimulation of individual cells of the various types found in and around the pacemaker tissue but also the recording of nonpropagated activity in cells stimulated or in those in the immediate vicinity. It was found that: 1) The myocardial atrial fiber has a threshold of the order of 10
−6 A. when stimulated intracellulary with a 15 msec duration pulse. This threshold was constant throughout diastole, except during its very early phase. The resulting response was of an all-or-none type and propagated over the tissue. 2) Thresholds for latent pacemaker cells were different from cell to cell; within a cell, it also varied slightly with the degree of diastolic depolarization attained. The response initiated by intracellular stimulation was also of an all-or-none type and, in most cases, it was propagated to the atrium. When a response was induced at an early phase in diastole, a conduction failure was sometimes observed. When such a nonpropagated response occurred in a latent pacemaker cell, the duration of diastole was increased. 3) Most pacemaker cells tested did not respond to intracellular stimulation. In a few cells, however, a graded response proportional to stimulus intensity, was obtained. Such response did not propagate to the atrium. These graded responses differed from the local responses which occur in other types of cell. It was concluded that a synchronization of pacemaker cell activity is required for effective pacemaker action. |
---|---|
AbstractList | The mechanism of pacemaker action in an isolated rabbit sinoatrial node preparation was studied by means of intracellular stimulation and recording through a single-barrelled microelectrode.
This technique permitted not only stimulation of individual cells of the various types found in and around the pacemaker tissue but also the recording of nonpropagated activity in cells stimulated or in those in the immediate vicinity. It was found that: 1) The myocardial atrial fiber has a threshold of the order of 10
−6 A. when stimulated intracellulary with a 15 msec duration pulse. This threshold was constant throughout diastole, except during its very early phase. The resulting response was of an all-or-none type and propagated over the tissue. 2) Thresholds for latent pacemaker cells were different from cell to cell; within a cell, it also varied slightly with the degree of diastolic depolarization attained. The response initiated by intracellular stimulation was also of an all-or-none type and, in most cases, it was propagated to the atrium. When a response was induced at an early phase in diastole, a conduction failure was sometimes observed. When such a nonpropagated response occurred in a latent pacemaker cell, the duration of diastole was increased. 3) Most pacemaker cells tested did not respond to intracellular stimulation. In a few cells, however, a graded response proportional to stimulus intensity, was obtained. Such response did not propagate to the atrium. These graded responses differed from the local responses which occur in other types of cell. It was concluded that a synchronization of pacemaker cell activity is required for effective pacemaker action. |
Author | Ushiyama, Junji Brooks, Chandler McC |
Author_xml | – sequence: 1 givenname: Junji surname: Ushiyama fullname: Ushiyama, Junji – sequence: 2 givenname: Chandler McC surname: Brooks fullname: Brooks, Chandler McC |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/4822536$$D View this record in MEDLINE/PubMed |
BookMark | eNqFkMlKBDEQhoMoOjP6CEJOoofWLL2kTyKDGwgeXPAWslRrpDutSVrw7c04g1cPRSqp_69KfXO07UcPCB1SckoJrc8eCGGsIA2vj5vyROQbLV620IxWnBWi5GQbzf4ke2ge4zshpGUN20W7pWCs4vUMPd_6FJSBvp96FXBMbshJcqPHylscwIzBOv-KuzAOOOasB2xUflMGr2wRO4_TG-CgtHYJqxTcNOyjnU71EQ425wI9XV0-Lm-Ku_vr2-XFXWF4TVKhTWdr24JuGyNytGWj2qYT1ILVoq6NNR1kaUWEMtQyzWkH2opKGE51BXyBjtZ9P8L4OUFMcnBx9S3lYZyiFIzzltU8C6u10IQxxgCd_AhuUOFbUiJXPOUvT7mCJZtS_vKUL9l3uBkw6QHsn2sDMNfP13XIW345CDIaB96AdZldknZ0_0z4AbZGiSE |
CitedBy_id | crossref_primary_10_1016_S0022_0736_77_80029_0 crossref_primary_10_1016_0002_9149_81_90069_2 crossref_primary_10_1126_science_16342 crossref_primary_10_1016_S0022_0736_77_80050_2 |
Cites_doi | 10.1016/0002-9149(62)90246-1 10.2170/jjphysiol.9.419 10.1007/BF00341523 10.2170/jjphysiol.13.231 10.1113/jphysiol.1951.sp004667 |
ContentType | Journal Article |
Copyright | 1974 Research in Electrocardiology, Inc. |
Copyright_xml | – notice: 1974 Research in Electrocardiology, Inc. |
DBID | CGR CUY CVF ECM EIF NPM AAYXX CITATION 7X8 |
DOI | 10.1016/S0022-0736(74)80021-X |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef MEDLINE - Academic |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef MEDLINE - Academic |
DatabaseTitleList | |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Medicine |
EISSN | 1532-8430 |
EndPage | 126 |
ExternalDocumentID | 10_1016_S0022_0736_74_80021_X 4822536 S002207367480021X |
Genre | Journal Article |
GroupedDBID | --- --K --M .1- .55 .FO .GJ .~1 0R~ 1B1 1P~ 1RT 1~. 1~5 29K 3V. 4.4 457 4G. 53G 5GY 5RE 5VS 7-5 71M 7X7 88E 88I 8AF 8AO 8FI 8FJ 8P~ 8R4 8R5 9JM AACTN AAEDT AAEDW AAIAV AAIKJ AAKOC AALRI AAOAW AAQFI AAQXK AAWTL AAXUO ABBQC ABFRF ABLVK ABMAC ABMZM ABOCM ABUWG ABXDB ABYKQ ACDAQ ACGFO ACGFS ACGOD ACRLP ADBBV ADEZE ADMUD AEBSH AEFWE AEKER AENEX AEVXI AFCTW AFFNX AFKRA AFKWA AFRHN AFTJW AFXIZ AGHFR AGUBO AGYEJ AHHHB AHMBA AHPSJ AIEXJ AIKHN AITUG AJBFU AJOXV AJRQY AJUYK ALMA_UNASSIGNED_HOLDINGS AMFUW AMRAJ ANZVX ASPBG AVWKF AXJTR AZFZN AZQEC BENPR BKOJK BLXMC BNPGV BPHCQ BVXVI CAG CCPQU COF CS3 D-I DU5 DWQXO EBS EFJIC EFLBG EJD EO8 EO9 EP2 EP3 F5P FDB FEDTE FGOYB FIRID FNPLU FYGXN FYUFA G-2 G-Q GBLVA GNUQQ HCIFZ HEB HMCUK HMK HMO HVGLF HZ~ IHE J1W K-O KOM L7B LCYCR M1P M29 M2P M2Q M41 MJL MO0 N9A O-L O9- OAUVE OA~ OL0 OZT P-8 P-9 P2P PC. PQQKQ PROAC PSQYO Q2X Q38 R2- RIG ROL RPZ RWL S0X SAE SDF SDG SEL SES SEW SPCBC SSH SSZ T5K TAE UKHRP UNMZH WH7 WUQ X7M Z5R ZA5 ZGI ~G- AKRWK CGR CUY CVF ECM EIF NPM AAXKI AAYXX ALIPV CITATION 7X8 |
ID | FETCH-LOGICAL-c360t-bcfd6d9eb97c897c947a97f81dedb866cdcfec36508ac1d2b31febd858c31b5e3 |
ISSN | 0022-0736 |
IngestDate | Fri Aug 16 08:04:58 EDT 2024 Thu Sep 12 16:55:55 EDT 2024 Thu May 23 23:52:55 EDT 2024 Fri Feb 23 02:30:12 EST 2024 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 2 |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-c360t-bcfd6d9eb97c897c947a97f81dedb866cdcfec36508ac1d2b31febd858c31b5e3 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
PMID | 4822536 |
PQID | 82339263 |
PQPubID | 23479 |
PageCount | 8 |
ParticipantIDs | proquest_miscellaneous_82339263 crossref_primary_10_1016_S0022_0736_74_80021_X pubmed_primary_4822536 elsevier_sciencedirect_doi_10_1016_S0022_0736_74_80021_X |
PublicationCentury | 1900 |
PublicationDate | 1974 1974-00-00 1974-1-00 19740101 |
PublicationDateYYYYMMDD | 1974-01-01 |
PublicationDate_xml | – year: 1974 text: 1974 |
PublicationDecade | 1970 |
PublicationPlace | United States |
PublicationPlace_xml | – name: United States |
PublicationTitle | Journal of electrocardiology |
PublicationTitleAlternate | J Electrocardiol |
PublicationYear | 1974 |
Publisher | Elsevier Inc |
Publisher_xml | – name: Elsevier Inc |
References | Hoffman (bib3) 1961 West (bib10) 1961 Cranefield, Hoffman (bib2) 1958 Trautwein, Uchizuno (bib5) 1963; 61 Brooks, Lu (bib1) 1972 Ushiyama, Lu, Lange, Brooks (bib8) 1964; 7 Matsuda, Hoshi, Kameyama (bib4) 1958; 9 Ushiyama, Brooks (bib7) 1963; 13 Weidmann (bib9) 1951; 115 Ushiyama, Brooks (bib6) 1962; 10 Ushiyama (10.1016/S0022-0736(74)80021-X_bib8) 1964; 7 Cranefield (10.1016/S0022-0736(74)80021-X_bib2) 1958 Matsuda (10.1016/S0022-0736(74)80021-X_bib4) 1958; 9 Ushiyama (10.1016/S0022-0736(74)80021-X_bib7) 1963; 13 Brooks (10.1016/S0022-0736(74)80021-X_bib1) 1972 Weidmann (10.1016/S0022-0736(74)80021-X_bib9) 1951; 115 West (10.1016/S0022-0736(74)80021-X_bib10) 1961 Hoffman (10.1016/S0022-0736(74)80021-X_bib3) 1961 Ushiyama (10.1016/S0022-0736(74)80021-X_bib6) 1962; 10 Trautwein (10.1016/S0022-0736(74)80021-X_bib5) 1963; 61 |
References_xml | – start-page: 38 year: 1958 end-page: 41 ident: bib2 article-title: Electrophysiology of single cardiac cells publication-title: Physiol Rev contributor: fullname: Hoffman – volume: 9 start-page: 419 year: 1958 ident: bib4 article-title: Effects of acconitine on the cardiac membrane potential of the dog publication-title: Jap J Physiol contributor: fullname: Kameyama – volume: 7 start-page: 275 year: 1964 ident: bib8 article-title: Recovery of intrinsic activity in isolated pacemakers following overdrive publication-title: Physiologist contributor: fullname: Brooks – start-page: 81 year: 1961 ident: bib10 article-title: Effect of chronotropic influences on subthreshold oscillations in the sino-atrial node publication-title: The Specialized Tissues of the Heart contributor: fullname: West – start-page: 143 year: 1961 ident: bib3 article-title: Electrical activity of the atrio-ventricular node publication-title: The Specialized Tissues of the Heart contributor: fullname: Hoffman – volume: 13 start-page: 231 year: 1963 ident: bib7 article-title: The safety factor for conduction in cardiac muscle publication-title: Jap J Physiol contributor: fullname: Brooks – volume: 10 start-page: 688 year: 1962 ident: bib6 article-title: Intracellular stimulation and recording from single cardiac cells publication-title: Am J Cardiol contributor: fullname: Brooks – year: 1972 ident: bib1 article-title: The Sinoatrial Pacemaker of the Heart contributor: fullname: Lu – volume: 61 start-page: 96 year: 1963 ident: bib5 article-title: Electron microscopic and electrophysiologic study of the pacemaker in sino-atrial node of the rabbit heart publication-title: Zeitschrift für Zellforschung contributor: fullname: Uchizuno – volume: 115 start-page: 227 year: 1951 ident: bib9 article-title: Effect of current flow on the membrane potential of cardiac muscle publication-title: J Physiol contributor: fullname: Weidmann – volume: 10 start-page: 688 year: 1962 ident: 10.1016/S0022-0736(74)80021-X_bib6 article-title: Intracellular stimulation and recording from single cardiac cells publication-title: Am J Cardiol doi: 10.1016/0002-9149(62)90246-1 contributor: fullname: Ushiyama – volume: 9 start-page: 419 year: 1958 ident: 10.1016/S0022-0736(74)80021-X_bib4 article-title: Effects of acconitine on the cardiac membrane potential of the dog publication-title: Jap J Physiol doi: 10.2170/jjphysiol.9.419 contributor: fullname: Matsuda – volume: 7 start-page: 275 year: 1964 ident: 10.1016/S0022-0736(74)80021-X_bib8 article-title: Recovery of intrinsic activity in isolated pacemakers following overdrive publication-title: Physiologist contributor: fullname: Ushiyama – start-page: 143 year: 1961 ident: 10.1016/S0022-0736(74)80021-X_bib3 article-title: Electrical activity of the atrio-ventricular node contributor: fullname: Hoffman – volume: 61 start-page: 96 year: 1963 ident: 10.1016/S0022-0736(74)80021-X_bib5 article-title: Electron microscopic and electrophysiologic study of the pacemaker in sino-atrial node of the rabbit heart publication-title: Zeitschrift für Zellforschung doi: 10.1007/BF00341523 contributor: fullname: Trautwein – volume: 13 start-page: 231 year: 1963 ident: 10.1016/S0022-0736(74)80021-X_bib7 article-title: The safety factor for conduction in cardiac muscle publication-title: Jap J Physiol doi: 10.2170/jjphysiol.13.231 contributor: fullname: Ushiyama – volume: 115 start-page: 227 year: 1951 ident: 10.1016/S0022-0736(74)80021-X_bib9 article-title: Effect of current flow on the membrane potential of cardiac muscle publication-title: J Physiol doi: 10.1113/jphysiol.1951.sp004667 contributor: fullname: Weidmann – year: 1972 ident: 10.1016/S0022-0736(74)80021-X_bib1 article-title: The Sinoatrial Pacemaker of the Heart contributor: fullname: Brooks – start-page: 81 year: 1961 ident: 10.1016/S0022-0736(74)80021-X_bib10 article-title: Effect of chronotropic influences on subthreshold oscillations in the sino-atrial node contributor: fullname: West – start-page: 38 year: 1958 ident: 10.1016/S0022-0736(74)80021-X_bib2 article-title: Electrophysiology of single cardiac cells publication-title: Physiol Rev contributor: fullname: Cranefield |
SSID | ssj0009272 |
Score | 1.2224836 |
Snippet | The mechanism of pacemaker action in an isolated rabbit sinoatrial node preparation was studied by means of intracellular stimulation and recording through a... |
SourceID | proquest crossref pubmed elsevier |
SourceType | Aggregation Database Index Database Publisher |
StartPage | 119 |
SubjectTerms | Animals Atrial Function Differential Threshold Electric Stimulation Electrophysiology Heart Atria - cytology Heart Conduction System - cytology Heart Conduction System - physiology Microelectrodes Rabbits |
Title | Intracellular stimulation and recording from single cardiac cells in the rabbit atrium |
URI | https://dx.doi.org/10.1016/S0022-0736(74)80021-X https://www.ncbi.nlm.nih.gov/pubmed/4822536 https://search.proquest.com/docview/82339263 |
Volume | 7 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Zj9MwELZKV6B9QVwryukHVgKhLEmc2M4L0gqBFlbLA2yhb5aviAqarno8wK9nfCRNWSqOh0ZVEjvRzOfxzGQOhJ4QW2vYOmji-honBZEq4USxxFBqS5aaqvRdIs7e05Nx8W5STgaDl72opfVKHekfv80r-R-uwjngq8uS_QfOdpPCCfgP_IUjcBiOf8Xjt84z61zvPpYUFussNuMKUePesvSRki6HxDkFvrkoLwcJ_dwNW7ZRjgup1NQlNi6msTTDZX01Nszx47d88ePll-l3OZNbH5nAvJ9_DeULosPVhFy70C2nF-SfhqwA2CZa2QjCs4ifUaLwZD2M5D1BmAVBeElAB1_Bx27-Q1dv_zCvuI8VmfTHAK0vZp5zBWgxJfmlYrbfg-OVK2gvZ2DpDdHe8emHz6ebsss56-rFu8dtcrhebN7hKSuexefvo6txyl16yi47xOsj5zfQ9cgYfBxQcRMNbHMLXTuLoRK30actcOAeODCAA3fgwA4cOIADR3BgDw48bTCAAwdw4ACOO2j85vX5q5MkNtFINKHpKlG6NtRUVlVMc_hVBZMVq8FMsUZxSrXRtYVbQVGXOjO5IlltleEl1yRTpSUHaNjMG3sX4TyVsI7rNFOMFpmsONUWDAZLVclrSYsROmpJJi5CrRSxCSIEagtHbcEK4aktJiPEW8KKqPAFRU4AXP409HHLCAEC0ZFFNna-XgqeE9D5KRmhg8Cf7l0iZ-_tunAf7btVEBxsD9BwtVjbh6ByrtSjiKufzqJ8mA |
link.rule.ids | 315,786,790,4043,27956,27957,27958 |
linkProvider | Library Specific Holdings |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Intracellular+stimulation+and+recording+from+single+cardiac+cells+in+the+rabbit+atrium&rft.jtitle=Journal+of+electrocardiology&rft.au=Ushiyama%2C+J&rft.au=Brooks%2C+C+M&rft.date=1974&rft.issn=0022-0736&rft.eissn=1532-8430&rft.volume=7&rft.issue=2&rft.spage=119&rft_id=info:doi/10.1016%2FS0022-0736%2874%2980021-X&rft_id=info%3Apmid%2F4822536&rft.externalDocID=4822536 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0022-0736&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0022-0736&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0022-0736&client=summon |