Rehydrated air-dried Pap smears as an alternative to wet-fixed smears
To assess the effect of rehydration on air-dried Pap smears and to compare them with conventional, wet-fixed smears. Paired cervical smears were obtained from 419 women. One set was labelled "Routine" (fixed immediately in 95% ethanol) and the other, "Rehydrated" (air-dried, rehy...
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Published in | Acta cytologica Vol. 46; no. 4; p. 713 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Switzerland
01.07.2002
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Abstract | To assess the effect of rehydration on air-dried Pap smears and to compare them with conventional, wet-fixed smears.
Paired cervical smears were obtained from 419 women. One set was labelled "Routine" (fixed immediately in 95% ethanol) and the other, "Rehydrated" (air-dried, rehydrated and then fixed). The latter were further subdivided into groups A-F depending on the duration of air drying. The staining quality of the smears was assessed, after adequate blinding to remove bias, and graded. The chi 2 test was performed to compare parameters in the two sets of smears.
Of the 397 pairs found to have adequate cellularity, 6% of the Routine group and 4% of the Rehydrated were "unsatisfactory due to poor fixation." Smears rehydrated within two hours of air drying as well as those refrigerated within two hours and rehydrated after 48 hours produced a staining quality as good as that of the conventional, wet-fixed smears. Rehydration caused lysis of red blood cells in the background.
Rehydration of air-dried Pap smears within two hours of preparation produces staining quality equal or superior to that of conventional, wet fixed ones. It has the added advantage of being less cumbersome for the clinical personnel involved in smear making. |
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AbstractList | To assess the effect of rehydration on air-dried Pap smears and to compare them with conventional, wet-fixed smears.
Paired cervical smears were obtained from 419 women. One set was labelled "Routine" (fixed immediately in 95% ethanol) and the other, "Rehydrated" (air-dried, rehydrated and then fixed). The latter were further subdivided into groups A-F depending on the duration of air drying. The staining quality of the smears was assessed, after adequate blinding to remove bias, and graded. The chi 2 test was performed to compare parameters in the two sets of smears.
Of the 397 pairs found to have adequate cellularity, 6% of the Routine group and 4% of the Rehydrated were "unsatisfactory due to poor fixation." Smears rehydrated within two hours of air drying as well as those refrigerated within two hours and rehydrated after 48 hours produced a staining quality as good as that of the conventional, wet-fixed smears. Rehydration caused lysis of red blood cells in the background.
Rehydration of air-dried Pap smears within two hours of preparation produces staining quality equal or superior to that of conventional, wet fixed ones. It has the added advantage of being less cumbersome for the clinical personnel involved in smear making. |
Author | Sivaraman, Ganesan Iyengar, Krishnan R |
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BackLink | https://www.ncbi.nlm.nih.gov/pubmed/12146037$$D View this record in MEDLINE/PubMed |
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CitedBy_id | crossref_primary_10_1016_j_jhin_2004_06_022 crossref_primary_10_1097_00006250_200310000_00018 crossref_primary_10_1016_j_jpha_2012_02_008 crossref_primary_10_1159_000351362 crossref_primary_10_25259_DJIGIMS_9_2023 crossref_primary_10_18231_j_jooo_2021_059 crossref_primary_10_4103_joc_joc_98_23 crossref_primary_10_1159_000530924 |
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References | 14674098 - Acta Cytol. 2003 Nov-Dec;47(6):1139-40 |
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Title | Rehydrated air-dried Pap smears as an alternative to wet-fixed smears |
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