Glycosphingolipids of skeletal muscle: I. Subcellular distribution of neutral glycosphingolipids and gangliosides in rabbit skeletal muscle

• Published in: Carbohydrate research Vol. 307; no. 1; pp. 135 - 145
• Main Authors: Müthing, Johannes, Maurer, Ulrich, Neumann, Ulrich, Kniep, Bernhard, Weber-Schürholz, Sabine
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01.02.1998
• Subjects: Animals; Antibodies; Antigens, CD; Carbohydrate Conformation; Carbohydrate Sequence; Cell Fractionation; Chromatography, Thin Layer; G(M1) Ganglioside - analysis; Gangliosides - analysis; Gangliosides - chemistry; Glucosylceramides - analysis; Glycosphingolipids - analysis; Glycosphingolipids - chemistry; Immunohistochemistry; Intracellular Membranes - chemistry; Lactosylceramides - analysis; Membrane fractions; Microtubules - chemistry; Mitochondria, Muscle - chemistry; Molecular Sequence Data; Muscle, Skeletal - chemistry; Muscle, Skeletal - ultrastructure; Rabbits; Sarcolemma - chemistry; Sarcoplasmic Reticulum - chemistry; Subcellular localization; TLC immunostaining; Immunohistochemistry; Antibodies; TLC immunostaining; Membrane fractions; Subcellular localization

Membrane vesicles were prepared from rabbit skeletal muscle, separated by sucrose density gradient centrifugation and characterized by their specific marker enzymes, ligand binding, and ion flux activities. The fractions obtained (in the order of increasing density) were sarcolemma (SL), T-tubules (...