TNF- α promoter polymorphisms, production and susceptibility to multiple sclerosis in different groups of patients
TNF- α production in whole blood cultures upon stimulation with LPS was determined in 179 individuals from 61 families in order to characterise the magnitude of inherited differences in TNF- α production. The three families characterised by highest TNF production showed 7.1±0.3 ng TNF/ml upon cultur...
Saved in:
Published in | Journal of neuroimmunology Vol. 72; no. 2; pp. 149 - 153 |
---|---|
Main Authors | , , , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
Elsevier B.V
01.02.1997
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | TNF-
α production in whole blood cultures upon stimulation with LPS was determined in 179 individuals from 61 families in order to characterise the magnitude of inherited differences in TNF-
α production. The three families characterised by highest TNF production showed 7.1±0.3 ng TNF/ml upon culture with 10 ng LPS and 10.2±0.2 ng TMF/ml upon culture with 1000 ng LPS, in contrast to the three families characterised by the lowest TNF production that showed a production of 1.6±0.1 ng TNF upon culture with 10 ng and 2.5±0.2 ng/ml upon culture with 1000 ng LPS/ml. This difference could not be attributed to the promoter polymorphisms −308 G to A, −238 G to A or −376 G to A, although the −238 GA donors produced 2.1±0.9 ng TNF upon culture with 10 ng endotoxin compared to 3.2±2.2 ng TNF for the −238 GG donors. In line with these results the frequency of the −238 GG genotype was increased in hospitalized MS patients in a nursing home (100% 238GG,
n=57) compared to MS patients in an outpatient's clinic (94% 238GG,
n=98) or Dutch controls (90% 238GG,
n=180). These results suggest that the −238 GG genotype is differently distributed in hospitalized MS patients in a nursing home. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0165-5728 1872-8421 |
DOI: | 10.1016/S0165-5728(96)00182-8 |