Partial purification of cysteine sulfinic acid decarboxylase from calf brain

• Published in: Acta chemica Scandinavica. Series B. Organic chemistry and biochemistry Vol. 34; no. 5; p. 363
• Main Authors: Heinämäki, A A, Piha, R S
• Format: Journal Article
• Language: English
• Published: Denmark 1980
• Subjects: Animals; Brain - enzymology; Carboxy-Lyases - isolation & purification; Carboxy-Lyases - metabolism; Cattle; Hydrogen-Ion Concentration; Kinetics; Molecular Weight; Substrate Specificity

Cysteine sulfinic acid decarboxylase (EC 4.1.1.29) was purified from calf brain by pH precipitation, ammonium sulfate fractionation, gel filtration and DEAE-Sephadex A-50 chromatography. The enzyme preparation decarboxylated both cysteine sulfinic acid and cysteic acid at all steps of the purificati...