Molecular detection of Cooperia oncophora and Ostertagia ostertagi in cattle: Comparison of sample processing and detection on ddPCR and qPCR platforms

• Published in: Experimental parasitology Vol. 268; p. 108878
• Main Authors: Woolsey, Ian David, Opsal, Tonje, Robertson, Lucy, Ptochos, Sokratis, Hektoen, Lisbeth
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.01.2025
• Subjects: Cooperia oncophora; Droplet digital PCR; Ostertagia ostertagi; Quantitative PCR; Droplet digital PCR; Ostertagia ostertagi; Quantitative PCR; Cooperia oncophora

Faecal samples were obtained from 77 first season grazers from 20 Norwegian dairy herds in autumn 2020 for analysis of Cooperia oncophora and Ostertagia ostertagi infection. Strongylid eggs per gram of faeces (EPG) were determined for each sample and the samples underwent larval culture. DNA was ext...