Characterization of NTPDase (NTPDase1; ecto-apyrase; ecto-diphosphohydrolase; CD39; EC 3.6.1.5) activity in human lymphocytes

• Published in: Biochimica et biophysica acta Vol. 1721; no. 1; pp. 9 - 15
• Main Authors: Leal, Daniela B.R., Streher, Cristiane A., Neu, Tiago N., Bittencourt, Fábio P., Leal, Cláudio A.M., da Silva, José E.P., Morsch, Vera M., Schetinger, Maria R.C.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 18.01.2005
• Subjects: Adenosine Diphosphate - metabolism; Adenosine Triphosphatases - metabolism; Adenosine Triphosphate - metabolism; Antigens, CD - metabolism; Apyrase - metabolism; CD39; Human lymphocyte; Humans; Kinetics; Lymphocytes - enzymology; NTPDase; Substrate Specificity; CD39; NTPDase; Human lymphocyte
• ISSN: 0304-4165; 0006-3002; 1872-8006
• DOI: 10.1016/j.bbagen.2004.09.006

Human lymphocytes contain NTPDase (NTPDase-1; ecto-apyrase; ecto-diphosphohydrolase; CD39; EC 3.6.1.5), a cation-dependent enzyme that hydrolyzes ATP and ADP and also other di- and triphosphate nucleosides, acting at an optimum pH of 8.0. A significant inhibition of ATP and ADP hydrolysis ( P<0.0...