Identification, cloning and functional characterization of a novel dermonecrotic toxin (phospholipase D) from brown spider (Loxosceles intermedia) venom

• Published in: Biochimica et biophysica acta Vol. 1780; no. 2; pp. 167 - 178
• Main Authors: Appel, Marcia Helena, da Silveira, Rafael Bertoni, Chaim, Olga Meiri, Paludo, Kátia Sabrina, Silva, Dilza Trevisan, Chaves, Daniele M., da Silva, Paulo Henrique, Mangili, Oldemir C., Senff-Ribeiro, Andrea, Gremski, Waldemiro, Nader, Helena B., Veiga, Silvio Sanches
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2008
• Subjects: Amino Acid Sequence; Animals; Capillary Permeability - drug effects; Cloning, Molecular; Dermis - drug effects; Dermis - pathology; Dermonecrotic toxin; DNA, Complementary - genetics; Edema - chemically induced; Loxosceles; Mice; Molecular Sequence Data; Necrosis - chemically induced; Phospholipase D; Phospholipase D - chemistry; Phospholipase D - genetics; Phospholipase D - toxicity; Phosphoric Diester Hydrolases - genetics; Phosphoric Diester Hydrolases - toxicity; Phylogeny; Protein Isoforms - chemistry; Protein Isoforms - genetics; Protein Isoforms - toxicity; Rabbits; Recombinant Proteins - chemistry; Recombinant Proteins - genetics; Recombinant Proteins - toxicity; Sphingomyelinase; Spider Venoms - chemistry; Spider Venoms - enzymology; Spider Venoms - genetics; Spider Venoms - toxicity; Spiders - enzymology; Venom; Sphingomyelinase; Loxosceles; Dermonecrotic toxin; Venom; Phospholipase D

Brown spider bites are associated with lesions including dermonecrosis, gravitational spreading and a massive inflammatory response, along with systemic problems that may include hematological disturbances and renal failure. The mechanisms by which the venom exerts its noxious effects are currently...