Bowman–Birk protease inhibitor from the seeds of Vigna unguiculata forms a highly stable dimeric structure
Different protease inhibitors including Bowman–Birk type (BBI) have been reported from the seeds of Vigna unguiculata. Protease isoinhibitors of double-headed Bowman–Birk type from the seeds of Vigna unguiculata have been purified and characterized. The BBI from Vigna unguiculata ( Vu-BBI) has been...
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Published in | Biochimica et biophysica acta Vol. 1774; no. 10; pp. 1264 - 1273 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Netherlands
Elsevier B.V
01.10.2007
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Subjects | |
Online Access | Get full text |
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Summary: | Different protease inhibitors including Bowman–Birk type (BBI) have been reported from the seeds of
Vigna unguiculata. Protease isoinhibitors of double-headed Bowman–Birk type from the seeds of
Vigna unguiculata have been purified and characterized. The BBI from
Vigna unguiculata (
Vu-BBI) has been found to undergo self-association to form very stable dimers and more complex oligomers, by size-exclusion chromatography and SDS-PAGE in the presence of urea. Many BBIs have been reported to undergo self-association to form homodimers or more complex oligomers in solution. Only one dimeric crystal structure of a BBI (pea-BBI) is reported to date. We report the three-dimensional structure of a
Vu-BBI determined at 2.5 Å resolution. Although, the inhibitor has a monomer fold similar to that found in other known structures of Bowman–Birk protease inhibitors, its quaternary structure is different from that commonly observed in this family. The structural elements responsible for the stability of monomer molecule and dimeric association are discussed. The
Vu-BBI may use dimeric or higher quaternary association to maintain the physiological state and to execute its biological function. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1570-9639 0006-3002 1878-1454 1878-2434 |
DOI: | 10.1016/j.bbapap.2007.07.009 |