Protein glycosylation in pmt mutants of Saccharomyces cerevisiae. Influence of heterologously expressed cellobiohydrolase II of Trichoderma reesei and elevated levels of GDP-mannose and cis-prenyltransferase activity

• Published in: Biochimica et biophysica acta Vol. 1770; no. 5; pp. 774 - 780
• Main Authors: Górka-Nieć, Wioletta, Bańkowska, Renata, Palamarczyk, Grażyna, Krotkiewski, Hubert, Kruszewska, Joanna S.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.05.2007
• Subjects: Cellulose 1,4-beta-Cellobiosidase - genetics; Cellulose 1,4-beta-Cellobiosidase - metabolism; cis-prenyltransferase activity; GDP-mannose level; Glycosylation; Guanosine Diphosphate Mannose - genetics; Guanosine Diphosphate Mannose - metabolism; Mannosyltransferases - genetics; Mannosyltransferases - metabolism; Nucleotidyltransferases - genetics; Nucleotidyltransferases - metabolism; O-mannosylation; pmt mutants; Saccharomyces cerevisiae - enzymology; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - growth & development; Transferases - genetics; Transferases - metabolism; Trichoderma - enzymology; Trichoderma - genetics; O-mannosylation; cis-prenyltransferase activity; GDP-mannose level; pmt mutants

Protein O-mannosylation has been postulated to be critical for production and secretion of glycoproteins in fungi. Therefore, understanding the regulation of this process and the influence of heterologous expression of glycoproteins on the activity of enzymes engaged in O-glycosylation are of consid...