Site-directed mutagenesis of two aromatic residues lining the active site pocket of the yeast Ltp1 - Paoli, Paolo; Modesti, Alessandra; Magherini, Francesca; Gamberi, Tania; Caselli, Anna; Manao, Giampaolo; Raugei, Giovanni; Camici, Guido; Ramponi, Giampietro (Biochimica et biophysica acta, 2007)
Keywords: Alanine - metabolism; Amino Acid Substitution; Amino Acids, Aromatic - chemistry; Amino Acids, Aromatic - genetics; Aromatic residue; Binding Sites; Catalysis; Hydrogen-Ion Concentration; Kinetics; LMW-PTP; Ltp1; Models, Molecular; Mutagenesis; Mutagenesis, Site-Directed; Phosphorylation; Protein Tyrosine Phosphatases - chemistry; Protein Tyrosine Phosphatases - genetics; PTP; Saccharomyces cerevisiae - chemistry; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae Proteins - chemistry; Saccharomyces cerevisiae Proteins - genetics; Substrate Specificity - genetics; Tryptophan - chemistry; Tryptophan - genetics; Tyrosine - chemistry; Tyrosine - genetics; Tyrosine phosphorylation; Yeast; CD; Aromatic residue; Yeast; Mutagenesis; Tyrosine phosphorylation; PTK; LMW; GST; pNPP; LMW-PTP; PTP; Ltp1