Purification and characterization of a lectin from the white shrimp Litopenaeus setiferus ( Crustacea decapoda) hemolymph

• Published in: Biochimica et biophysica acta Vol. 1724; no. 1; pp. 86 - 93
• Main Authors: Alpuche, Juan, Pereyra, Ali, Agundis, Concepción, Rosas, Carlos, Pascual, Cristina, Slomianny, Marie-Christine, Vázquez, Lorena, Zenteno, Edgar
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 20.06.2005
• Subjects: Animals; Carbohydrates - chemistry; Cations, Divalent - pharmacology; Crustaceans; Hemagglutination - drug effects; Hemocyanin; Hemolymph - chemistry; Hemolymph - metabolism; Lectins; Lectins - chemistry; Lectins - isolation & purification; Litopenaeus setiferus; MALDI-TOF; Penaeidae - metabolism; Sialic acid; Specific lectin; Crustaceans; Sialic acid; MALDI-TOF; Hemocyanin; Litopenaeus setiferus; Lectins; Specific lectin

A 291-kDa lectin (LsL) was purified from the hemolymph of the white shrimp Litopenaeus setiferus by affinity chromatography on glutaraldehyde-fixed stroma from rabbit erythrocytes. LsL is a heterotetramer of two 80-kDa and two 52-kDa subunits, with no covalently-liked carbohydrate, and mainly compos...