Temporal changes in charge content of cultured chondrocytes from bovine cartilaginous tissues

• Published in: Matrix biology Vol. 15; no. 7; pp. 495 - 502
• Main Authors: Van Damme, M.-P.I., Sinnaya, P., Derry, K., Murphy, W.H., Preston, B.N.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.03.1997
• Subjects: Animals; Anions - chemistry; Anions - metabolism; Cartilage - cytology; Cartilage, Articular - cytology; Cartilage, Articular - metabolism; Cattle; Cells, Cultured; Charge; chondrocytes; Colloids; Extracellular Matrix - chemistry; Extracellular Matrix - metabolism; Humans; Protein Biosynthesis; proteoglycans; proteoglycans; Charge; chondrocytes

The effective charge content of the pericellular matrix of chondrocytes has been determined while the matrix is being synthesized by cells grown in culture for several weeks. The data were compared with estimates determined by chemical analysis. When measurements were performed after digestion of th...