Proton NMR studies of ternary complexes of bleomycin with metal ions and nucleic acid

The formation of a ternary complex of bleomycin of the type metal ion-Bleo-A 2-poly(dA-dT) is indicated by the observation of spin diffusion in the 1H NMR spectra when nuclear Overhauser experiments are performed on samples containing poly(dA-dT) and Zn(II)-Bleo-A 2 or CO-Fe(II)-Bleo-A 2. The enhanc...

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Published inBiochemical and biophysical research communications Vol. 97; no. 1; pp. 270 - 278
Main Authors Pillai, Rajasekharan P., Rama Krishna, N., Sakai, Ted T., Glickson, Jerry D.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.01.1980
Subjects
Bleomycin
Chemical Phenomena
Chemistry
Iron
Magnetic Resonance Spectroscopy
Molecular Conformation
Nucleic Acid Conformation
Poly dA-dT
Polydeoxyribonucleotides
Protein Binding
Protein Conformation
Zinc
TSP
Bleo-A 2
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Summary:The formation of a ternary complex of bleomycin of the type metal ion-Bleo-A 2-poly(dA-dT) is indicated by the observation of spin diffusion in the 1H NMR spectra when nuclear Overhauser experiments are performed on samples containing poly(dA-dT) and Zn(II)-Bleo-A 2 or CO-Fe(II)-Bleo-A 2. The enhancement of the transverse relaxation rates of the resonances of Fe(II)-Bleo-A 2 by poly(dA-dT) demonstrates the formation of the putative active complex Fe(II)-Bleo-A 2-poly(dA-dT). The absence of substantial changes in the chemical shifts suggests that the geometry of metal ion co-ordination is minimally perturbed by polynucleotide binding, which is consistent with the bifunctional nature of bleomycin.
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ISSN:0006-291X
1090-2104
DOI:10.1016/S0006-291X(80)80164-1