Disaccharides permeases: constituents of xylanolytic and mannanolytic systems of Aureobasidium pullulans

• Published in: Biochimica et biophysica acta Vol. 1425; no. 3; pp. 560 - 566
• Main Authors: Lubomı́r, Kremnický, Peter, Biely
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 27.11.1998
• Subjects: Aureobasidium pullulans; Disaccharides - metabolism; Mannans - metabolism; Mannobiose permease; Mannosidases - biosynthesis; Membrane Transport Proteins - biosynthesis; Methyl β- d-mannopyranoside; Methyl β- d-xylopyranoside; Mitosporic Fungi - enzymology; Polysaccharides - metabolism; Substrate Specificity; Transport; Xylobiose permease; Xylosidases - biosynthesis; β-1,4-Mannanase; β-1,4-Xylanase; β-Mannosidase; β-Xylosidase; β-Xylosidase; Methyl β- d-mannopyranoside; β-Mannosidase; Xylobiose permease; Aureobasidium pullulans; β-1,4-Xylanase; β-1,4-Mannanase; Mannobiose permease; Transport; Methyl β- d-xylopyranoside
• ISSN: 0304-4165; 0006-3002; 1872-8006
• DOI: 10.1016/S0304-4165(98)00112-3

Aureobasidium pullulans, a yeast-like microorganism was found to produce mannobiose permease and xylobiose permease, transporting β-1,4-mannobiose or β-1,4-xylobiose into the cells from extracellular media. Both permeases are induced by the same inducers as the corresponding hemicellulolytic enzyme systems. Mannobiose permease is induced by β-1,4-mannobiose or is formed in the cells growing on mannan (inducers of β-mannanolytic enzymes) and xylobiose permease is induced by d-xylose, β-1,4-xylobiose or during the growth on xylan (inducers of xylanolytic enzymes). The permeases are energy dependent, synthesized de novo and their activities are inhibited by d-glucose. Since mannobiose permease transports β-1,4-mannobiose, xylobiose permease appears to be less specific and transports β-1,4-mannobiose, β-1,4-xylobiose and methyl β- d-xylopyranoside. Methyl β- d-mannopyranoside or methyl β- d-xylopyranoside serve as less efficient inducers of the corresponding permeases than β-1,4-mannobiose or β-1,4-xylobiose.