Expression of phase I and phase II genes in mouse embryonic stem cells cultured in the presence of 2,3,7,8-tetrachlorodibenzo- para-dioxin

• Published in: Biochimica et biophysica acta Vol. 1780; no. 5; pp. 826 - 836
• Main Authors: Neri, Tui, Merico, Valeria, Garagna, Silvia, Redi, Carlo Alberto, Zuccotti, Maurizio
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.05.2008
• Subjects: Ahr gene battery; Animals; Antigens, Differentiation - genetics; Antigens, Differentiation - metabolism; Apoptosis - drug effects; Aryl Hydrocarbon Hydroxylases - genetics; Aryl Hydrocarbon Hydroxylases - metabolism; bcl-2-Associated X Protein - genetics; bcl-2-Associated X Protein - metabolism; Blotting, Western; Cell Differentiation - drug effects; Cell Line; Cytochrome P-450 CYP1A1 - genetics; Cytochrome P-450 CYP1A1 - metabolism; Cytochrome P-450 CYP1A2 - genetics; Cytochrome P-450 CYP1A2 - metabolism; Cytochrome P-450 CYP1B1; Dioxin; Dose-Response Relationship, Drug; Embryonic stem cell; Embryonic Stem Cells - drug effects; Embryonic Stem Cells - metabolism; Gene Expression - drug effects; Glucuronosyltransferase - genetics; Glucuronosyltransferase - metabolism; Glutathione Transferase - genetics; Glutathione Transferase - metabolism; Isoenzymes - genetics; Isoenzymes - metabolism; Kinetics; Mice; NAD(P)H Dehydrogenase (Quinone); NADPH Dehydrogenase - genetics; NADPH Dehydrogenase - metabolism; Polychlorinated Dibenzodioxins - pharmacology; Proto-Oncogene Proteins c-bcl-2 - genetics; Proto-Oncogene Proteins c-bcl-2 - metabolism; Receptors, Aryl Hydrocarbon - genetics; Receptors, Aryl Hydrocarbon - metabolism; Reverse Transcriptase Polymerase Chain Reaction; Time Factors; Embryonic stem cell; Ahr gene battery; Dioxin

Embryonic stem (ES) cells have features that resemble the pluripotent cells of peri-implantation embryos and have been used as an in vitro model to assess the effects of test substances on these stages of development. Here, for the first time, we report on the effects of the xenobiotic 2,3,7,8-tetra...