Functional characterization of the histidine kinase of the E. coli two-component signal transduction system AtoS–AtoC

• Published in: Biochimica et biophysica acta Vol. 1780; no. 9; pp. 1023 - 1031
• Main Authors: Filippou, Panagiota S., Kasemian, Lucy D., Panagiotidis, Christos A., Kyriakidis, Dimitrios A.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.09.2008
• Subjects: Adenosine Triphosphate - metabolism; Amino Acid Sequence; Antizyme; AtoC; atoDAEB; AtoS; Conserved Sequence; Cross-Linking Reagents - pharmacology; Dimerization; DNA-Binding Proteins - metabolism; Escherichia coli - drug effects; Escherichia coli - enzymology; Escherichia coli Proteins - chemistry; Escherichia coli Proteins - isolation & purification; Escherichia coli Proteins - metabolism; Glutaral - pharmacology; Histidine - metabolism; Histidine Kinase; Molecular Sequence Data; Mutant Proteins - metabolism; Mutation - genetics; Phosphates - metabolism; Phosphorylation; Phosphorylation - drug effects; Protein Kinases - chemistry; Protein Kinases - isolation & purification; Protein Kinases - metabolism; Recombinant Proteins - metabolism; Signal Transduction - drug effects; Two-component system; RR; atoSC; Two-component system; Phosphorylation; HK; TCS; AtoS; ODC; Histidine kinase; Antizyme; AtoC; Az; AcAc; atoDAEB

The Escherichia coli AtoS–AtoC two-component signal transduction system regulates the expression of the atoDAEB operon genes, whose products are required for short-chain fatty acid catabolism. In this study purified his-tagged wild-type and mutant AtoS proteins were used to prove that these proteins...